| pubmed-article:10799448 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0026724 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0086574 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0164209 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0205100 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:10799448 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:10799448 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:10799448 | pubmed:dateCreated | 2000-6-27 | lld:pubmed |
| pubmed-article:10799448 | pubmed:abstractText | Substance P (SP) has been implicated in peripheral and mucosal neuroimmunoregulation. However, confusion remains regarding immunocyte expression of the receptor for SP, neurokinin-1 receptor (NK-1R), and whether there is differential NK-1R expression in the mucosal versus the peripheral immune system. In the same assay systems, we examined the expression of NK-1R in human lamina propria mononuclear cells (LPMC), peripheral blood mononuclear cells (PBMC), peripheral blood lymphocytes (PBL), monocytes, and monocyte-derived macrophages (MDM). Using standard reverse transcription (RT)-PCR, mRNA expression of both the long and the short isoforms of the NK-1R was evident in LPMC but not in PBMC, PBL, monocytes, or MDM. However, by using nested RT-PCR NK-1R mRNA expression was detected in PBMC, PBL, monocytes, and MDM. This level of expression was found to represent one NK-1R mRNA transcript in >1,000 cells. In contrast, by using competitive RT-PCR we demonstrate that LPMC express a more biologically significant level of eight NK-1R mRNA transcripts per cell. Flow cytometric detection of NK-1R expression at the protein level was evident in LPMC but not in PBMC. These findings illustrate the extreme sensitivity of nested RT-PCR and the advantages of competitive RT-PCR in comparative studies of receptor expression in different cell populations. This study suggests that, under normal conditions, readily detectable expression of NK-1R in human mononuclear cells occurs at the mucosal level rather than in the peripheral circulation. | lld:pubmed |
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| pubmed-article:10799448 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10799448 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10799448 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10799448 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10799448 | pubmed:month | May | lld:pubmed |
| pubmed-article:10799448 | pubmed:issn | 1071-412X | lld:pubmed |
| pubmed-article:10799448 | pubmed:author | pubmed-author:CollinsJ KJK | lld:pubmed |
| pubmed-article:10799448 | pubmed:author | pubmed-author:DouglasS DSD | lld:pubmed |
| pubmed-article:10799448 | pubmed:author | pubmed-author:ShanahanFF | lld:pubmed |
| pubmed-article:10799448 | pubmed:author | pubmed-author:O'ConnellJJ | lld:pubmed |
| pubmed-article:10799448 | pubmed:author | pubmed-author:SaiMM | lld:pubmed |
| pubmed-article:10799448 | pubmed:author | pubmed-author:O'SullivanG... | lld:pubmed |
| pubmed-article:10799448 | pubmed:author | pubmed-author:GoodeTT | lld:pubmed |
| pubmed-article:10799448 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10799448 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:10799448 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10799448 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10799448 | pubmed:pagination | 371-6 | lld:pubmed |
| pubmed-article:10799448 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:10799448 | pubmed:meshHeading | pubmed-meshheading:10799448... | lld:pubmed |
| pubmed-article:10799448 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10799448 | pubmed:articleTitle | Differential expression of neurokinin-1 receptor by human mucosal and peripheral lymphoid cells. | lld:pubmed |
| pubmed-article:10799448 | pubmed:affiliation | Department of Medicine, National University of Ireland, Cork, Ireland. | lld:pubmed |
| pubmed-article:10799448 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10799448 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:10799448 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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