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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-6-13
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pubmed:abstractText |
We found that in MCF-7 breast carcinoma cells, PI3K and Akt suppressed a dose-dependent induction of apoptosis by tumor necrosis factor alpha (TNF). PI3K and Akt stimulated NF-kappaB activation in a dose-dependent manner, suggesting a common link between these two pathways. TNF has been shown to activate both an apoptotic cascade, as well as a cell survival signal through NF-kappaB. PI3K and AKT cell survival signaling were correlated with increased TNF-stimulated NF-kappaB activity in MCF-7 cells. We demonstrate that while both TNFR1 and NIK are partially involved in Akt-induced NF-kappaB stimulation, a dominant negative IkappaBalpha completely blocked Akt-NF-kappaB cross-talk. PI3K-Akt signaling activated NF-kappaB through both TNFR signaling-dependent and -independent mechanisms, potentially representing a mechanism by which Akt functions to suppress apoptosis in cancer.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
342-5
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10799299-Apoptosis,
pubmed-meshheading:10799299-Cell Line,
pubmed-meshheading:10799299-Dose-Response Relationship, Drug,
pubmed-meshheading:10799299-Humans,
pubmed-meshheading:10799299-Luciferases,
pubmed-meshheading:10799299-NF-kappa B,
pubmed-meshheading:10799299-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:10799299-Plasmids,
pubmed-meshheading:10799299-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10799299-Proto-Oncogene Proteins,
pubmed-meshheading:10799299-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:10799299-Recombinant Proteins,
pubmed-meshheading:10799299-Signal Transduction,
pubmed-meshheading:10799299-Transfection,
pubmed-meshheading:10799299-Tumor Cells, Cultured,
pubmed-meshheading:10799299-Tumor Necrosis Factor-alpha
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pubmed:year |
2000
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pubmed:articleTitle |
PI3-K/AKT regulation of NF-kappaB signaling events in suppression of TNF-induced apoptosis.
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pubmed:affiliation |
Tulane-Xavier Center for Bioenvironmental Research, Department of Pharmacology, Tulane University Medical Center, New Orleans, LA 70112, USA. mburow@tcs.tulane.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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