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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2000-5-31
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pubmed:abstractText |
The LightCycler(TM) combines rapid amplification of nucleic acids in glass capillaries with melting curve analysis based on fluorescence resonance energy transfer for the sensitive detection of point mutations in various settings, such as drug resistance and hereditary diseases. Point mutations leading to an altered structure of lanosteroldemethylase, the target enzyme of the fungistatic azoles, are an important mechanism of acquired resistance in Candida albicans.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0009-9147
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
631-5
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10794744-Acquired Immunodeficiency Syndrome,
pubmed-meshheading:10794744-Antifungal Agents,
pubmed-meshheading:10794744-Candida,
pubmed-meshheading:10794744-DNA, Fungal,
pubmed-meshheading:10794744-Electrophoresis, Agar Gel,
pubmed-meshheading:10794744-Fluconazole,
pubmed-meshheading:10794744-Humans,
pubmed-meshheading:10794744-Microbial Sensitivity Tests,
pubmed-meshheading:10794744-Point Mutation,
pubmed-meshheading:10794744-Polymerase Chain Reaction,
pubmed-meshheading:10794744-Reproducibility of Results,
pubmed-meshheading:10794744-Sensitivity and Specificity,
pubmed-meshheading:10794744-Spectrometry, Fluorescence
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pubmed:year |
2000
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pubmed:articleTitle |
Rapid detection of point mutations by fluorescence resonance energy transfer and probe melting curves in Candida species.
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pubmed:affiliation |
Medizinische Klinik, Abteilung II, Otfried-Mueller-Strasse 10, 72076 Tuebingen, Germany. Fax 49-7071-293179, USA. juergen.loeffler@med.uni-tuebingen.de
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pubmed:publicationType |
Journal Article
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