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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-5-16
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pubmed:abstractText |
Several electrophoretic and chromatographic systems have been investigated and compared for sequence analysis of oligodeoxyribonucleotides. Three systems were found to be useful for the separation of a series of sequential degradation products resulting from a labeled oligonucleotide: (I) 2-D electrophoresisdagger; (II) 2-D PEI-cellulose; and (III) 2-D homochromatography. System (III) proved generally most informative regardless of base composition and sequence. Furthermore, only in this system will the omission of an oligonucleotide in a series of oligonucleotides be self-evident from the two-dimensional map. The sequence of up to fifteen nucleotides can be determined solely by the characteristic mobility shifts of its sequential degradation products distributed on the two-dimensional map. With this method, ten nucleotides from the double-stranded region adjacent to the left-hand 3'-terminus and seven from the right-hand 3'-terminus of bacteriophage lambda DNA have been sequenced. Similarly, nine nucleotides from the double-stranded region adjacent to the left-hand 3'-terminus and five nucleotides from the right-hand terminus of bacteriophage phi80 DNA have also been sequenced. The advantages and disadvantages of each separation system with respect to sequence analysis are discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4291729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4334766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4343968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4343974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4348308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4352720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4354250,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4358929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4501589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4515613,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4515740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4553110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4560009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4563439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4569877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4577794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4702874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4732072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4753202,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4902612,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4931680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-4942451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-5038449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-5125354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-5312501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-5325727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-5476726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10793670-6027247
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-53
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:10793670-Base Sequence,
pubmed-meshheading:10793670-Cellulose,
pubmed-meshheading:10793670-Chromatography, Thin Layer,
pubmed-meshheading:10793670-DNA, Viral,
pubmed-meshheading:10793670-Electrophoresis, Cellulose Acetate,
pubmed-meshheading:10793670-Electrophoresis, Gel, Two-Dimensional,
pubmed-meshheading:10793670-Oligodeoxyribonucleotides,
pubmed-meshheading:10793670-Polyethyleneimine,
pubmed-meshheading:10793670-Sequence Analysis, DNA
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pubmed:year |
1974
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pubmed:articleTitle |
DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.
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pubmed:publicationType |
Journal Article
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