Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-8-29
pubmed:abstractText
The psae1-1 mutant of Arabidopsis was identified on the basis of a decrease in the effective quantum yield of photosystem II, among a collection of plants subjected to transposon tagging with the Enhancer element. The steady-state redox level and the rate of re-oxidation of P700 are significantly altered in psae1-1 mutants. The responsible mutation was localised to psaE1, one of two Arabidopsis genes that encode subunit E of photosystem I. An additional mutant allele, psae1-2, was identified by reverse genetics. In wild-type plants, the psaE1 transcript is expressed at a higher level than psaE2 mRNA. In the mutants, however, the E1 transcript was barely detectable, and was expressed only in small groups of wild-type cells resulting from somatic reversions. As a consequence, the amount of PsaE protein present in the mutant is significantly reduced. Concomitantly, the levels of other stromal photosystem I subunits (PsaC and PsaD) are also affected. Mutant plants showed a marked increase in light sensitivity and photoinhibition. Additional effects of the psae1 mutation include light green pigmentation, an increase in chlorophyll fluorescence and a decrease of approximately 50% in growth rate under greenhouse conditions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10792827-Alleles, pubmed-meshheading:10792827-Amino Acid Sequence, pubmed-meshheading:10792827-Arabidopsis, pubmed-meshheading:10792827-Arabidopsis Proteins, pubmed-meshheading:10792827-Base Sequence, pubmed-meshheading:10792827-DNA Transposable Elements, pubmed-meshheading:10792827-Light-Harvesting Protein Complexes, pubmed-meshheading:10792827-Molecular Sequence Data, pubmed-meshheading:10792827-Mutagenesis, Insertional, pubmed-meshheading:10792827-Oxidation-Reduction, pubmed-meshheading:10792827-Photosynthesis, pubmed-meshheading:10792827-Photosynthetic Reaction Center Complex Proteins, pubmed-meshheading:10792827-Photosystem I Protein Complex, pubmed-meshheading:10792827-Photosystem II Protein Complex, pubmed-meshheading:10792827-Sequence Alignment, pubmed-meshheading:10792827-Sequence Analysis, DNA
pubmed:year
2000
pubmed:articleTitle
Disruption of the Arabidopsis photosystem I gene psaE1 affects photosynthesis and impairs growth.
pubmed:affiliation
Zentrum zur Identifikation von Genfunktionen durch Insertionsmutagenese bei Arabidopsis thaliana (ZIGIA), Max-Planck-Institut für Züchtungsforschung, Carl von Linné Weg 10, 50829 Köln, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't