| pubmed-article:10790110 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C0206558 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C0042338 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C0042769 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C0040078 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C0430054 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C1705053 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:10790110 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:10790110 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10790110 | pubmed:dateCreated | 2000-6-21 | lld:pubmed |
| pubmed-article:10790110 | pubmed:abstractText | A rapid phenotypic screening method for herpes simplex virus (HSV) and varicella-zoster virus (VZV) thymidine kinase (TK) genes was developed for monitoring acyclovir-resistant viruses. This method determines the biochemical phenotype of the TK polypeptide, which is synthesized in vitro from viral DNA using a procedure as follows. The TK gene of each sample virus strain is amplified and isolated under the control of a T7 promoter by PCR. The PCR products are transcribed with T7 RNA polymerase and translated in a rabbit reticulocyte lysate. Using this method, enzymatic characteristics and the size of the TK polypeptides encoding HSV and VZV DNA were defined in less than 2 days without virus isolation. The assay should be a powerful tool in monitoring drug-resistant viruses, especially in cases in which virus isolation is difficult. | lld:pubmed |
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| pubmed-article:10790110 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10790110 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10790110 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10790110 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10790110 | pubmed:month | May | lld:pubmed |
| pubmed-article:10790110 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:10790110 | pubmed:author | pubmed-author:SaikiTT | lld:pubmed |
| pubmed-article:10790110 | pubmed:author | pubmed-author:OgasawaraMM | lld:pubmed |
| pubmed-article:10790110 | pubmed:author | pubmed-author:AzumaMM | lld:pubmed |
| pubmed-article:10790110 | pubmed:author | pubmed-author:SuzutaniTT | lld:pubmed |
| pubmed-article:10790110 | pubmed:author | pubmed-author:NagamineMM | lld:pubmed |
| pubmed-article:10790110 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10790110 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:10790110 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10790110 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10790110 | pubmed:pagination | 1839-44 | lld:pubmed |
| pubmed-article:10790110 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:10790110 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10790110 | pubmed:articleTitle | Rapid phenotypic characterization method for herpes simplex virus and Varicella-Zoster virus thymidine kinases to screen for acyclovir-resistant viral infection. | lld:pubmed |
| pubmed-article:10790110 | pubmed:affiliation | Department of Microbiology, Asahikawa Medical College, Asahikawa, Japan. suzutani@asahikawa-med.ac.jp | lld:pubmed |
| pubmed-article:10790110 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10790110 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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