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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2000-6-21
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pubmed:abstractText |
A rapid phenotypic screening method for herpes simplex virus (HSV) and varicella-zoster virus (VZV) thymidine kinase (TK) genes was developed for monitoring acyclovir-resistant viruses. This method determines the biochemical phenotype of the TK polypeptide, which is synthesized in vitro from viral DNA using a procedure as follows. The TK gene of each sample virus strain is amplified and isolated under the control of a T7 promoter by PCR. The PCR products are transcribed with T7 RNA polymerase and translated in a rabbit reticulocyte lysate. Using this method, enzymatic characteristics and the size of the TK polypeptides encoding HSV and VZV DNA were defined in less than 2 days without virus isolation. The assay should be a powerful tool in monitoring drug-resistant viruses, especially in cases in which virus isolation is difficult.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-10358747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-10421406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-1315366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-1663576,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-1666496,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-1698324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-1848597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2155570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2543743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2543763,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2543985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2554768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2821890,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2847637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-2997472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-3013088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-6247969,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-6256650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-6289742,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10790110-9767251
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1839-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10790110-Acyclovir,
pubmed-meshheading:10790110-Animals,
pubmed-meshheading:10790110-Antiviral Agents,
pubmed-meshheading:10790110-Bacteriophage T7,
pubmed-meshheading:10790110-Base Sequence,
pubmed-meshheading:10790110-Cell Line,
pubmed-meshheading:10790110-Cercopithecus aethiops,
pubmed-meshheading:10790110-Drug Resistance, Microbial,
pubmed-meshheading:10790110-Herpesvirus 1, Human,
pubmed-meshheading:10790110-Herpesvirus 2, Human,
pubmed-meshheading:10790110-Herpesvirus 3, Human,
pubmed-meshheading:10790110-Humans,
pubmed-meshheading:10790110-Molecular Sequence Data,
pubmed-meshheading:10790110-Promoter Regions, Genetic,
pubmed-meshheading:10790110-Protein Biosynthesis,
pubmed-meshheading:10790110-Rabbits,
pubmed-meshheading:10790110-Sequence Alignment,
pubmed-meshheading:10790110-Sequence Homology, Nucleic Acid,
pubmed-meshheading:10790110-Thymidine Kinase,
pubmed-meshheading:10790110-Vero Cells
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pubmed:year |
2000
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pubmed:articleTitle |
Rapid phenotypic characterization method for herpes simplex virus and Varicella-Zoster virus thymidine kinases to screen for acyclovir-resistant viral infection.
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pubmed:affiliation |
Department of Microbiology, Asahikawa Medical College, Asahikawa, Japan. suzutani@asahikawa-med.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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