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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2000-6-30
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pubmed:abstractText |
Pokeweed antiviral protein (PAP), a ribosome-inactivating protein isolated from the leaves of Phytolacca americana, reveals potent antiviral activity against viruses or cytotoxic action against cells once inside the cytoplasm. Therefore PAP is a good candidate to be used as an immunotoxin. We constructed a bacterial expression plasmid encoding PAP as a fusion protein with gonadotropin-releasing hormone (GnRH), a neuropeptide with receptor sites on several gynaecologic tumors. The resulting recombinant toxin was produced in Escherichia coli and accumulated in inclusion bodies. After purification under denaturing conditions, renaturated GnRH-PAP shows an IC(50) of 3 nM on in vitro translation assays and selectively inhibits the growth of the GnRH receptor positive Ishikawa cell line (ID(50) of 15 nM); on the other hand, neither GnRH nor PAP alone had any effect.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
472
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
241-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10788619-Antineoplastic Agents,
pubmed-meshheading:10788619-Drug Screening Assays, Antitumor,
pubmed-meshheading:10788619-Gene Expression,
pubmed-meshheading:10788619-Gonadotropin-Releasing Hormone,
pubmed-meshheading:10788619-Humans,
pubmed-meshheading:10788619-Immunotoxins,
pubmed-meshheading:10788619-N-Glycosyl Hydrolases,
pubmed-meshheading:10788619-Plant Proteins,
pubmed-meshheading:10788619-Receptors, LHRH,
pubmed-meshheading:10788619-Recombinant Fusion Proteins,
pubmed-meshheading:10788619-Ribosome Inactivating Proteins, Type 1,
pubmed-meshheading:10788619-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Cytotoxic activity of a recombinant GnRH-PAP fusion toxin on human tumor cell lines.
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pubmed:affiliation |
Laboratoire de Biochimie et Biologie Moléculaire, Université de Franche-Comté, 16 route de Gray, 25030 Besançon, France. jlsk3@netcourrier.com
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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