Linked Life Data

10788342

Source:http://linkedlifedata.com/resource/pubmed/id/10788342

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-6-16
pubmed:abstractText
Forest and other upland soils are important sinks for atmospheric CH(4), consuming 20 to 60 Tg of CH(4) per year. Consumption of atmospheric CH(4) by soil is a microbiological process. However, little is known about the methanotrophic bacterial community in forest soils. We measured vertical profiles of atmospheric CH(4) oxidation rates in a German forest soil and characterized the methanotrophic populations by PCR and denaturing gradient gel electrophoresis (DGGE) with primer sets targeting the pmoA gene, coding for the alpha subunit of the particulate methane monooxygenase, and the small-subunit rRNA gene (SSU rDNA) of all life. The forest soil was a sink for atmospheric CH(4) in situ and in vitro at all times. In winter, atmospheric CH(4) was oxidized in a well-defined subsurface soil layer (6 to 14 cm deep), whereas in summer, the complete soil core was active (0 cm to 26 cm deep). The content of total extractable DNA was about 10-fold higher in summer than in winter. It decreased with soil depth (0 to 28 cm deep) from about 40 to 1 microg DNA per g (dry weight) of soil. The PCR product concentration of SSU rDNA of all life was constant both in winter and in summer. However, the PCR product concentration of pmoA changed with depth and season. pmoA was detected only in soil layers with active CH(4) oxidation, i.e., 6 to 16 cm deep in winter and throughout the soil core in summer. The same methanotrophic populations were present in winter and summer. Layers with high CH(4) consumption rates also exhibited more bands of pmoA in DGGE, indicating that high CH(4) oxidation activity was positively correlated with the number of methanotrophic populations present. The pmoA sequences derived from excised DGGE bands were only distantly related to those of known methanotrophs, indicating the existence of unknown methanotrophs involved in atmospheric CH(4) consumption.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-10049856, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-10427012, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-10473417, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-10667792, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-7590173, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-8017936, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-8801441, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-9464387, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-9501432, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-9588802, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-9647861, http://linkedlifedata.com/resource/pubmed/commentcorrection/10788342-9765151
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1801-8
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Molecular analyses of novel methanotrophic communities in forest soil that oxidize atmospheric methane.
pubmed:affiliation
Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Strasse, 35043 Marburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't