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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2000-5-11
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pubmed:abstractText |
The type I insulin-like growth factor receptor (IGF-IR) is known to send two seemingly contradictory signals inducing either cell proliferation or cell differentiation, depending on cell type and/or conditions. H19-7 cells are rat hippocampal neuronal cells immortalized by a temperature-sensitive SV40 large T antigen that grow at 34 degrees C in epidermal growth factor or serum but differentiate at 39 degrees C when induced by basic fibroblast growth factor. At 39 degrees C, expression of the human IGF-IR in H19-7 cells induces an insulin-like growth factor (IGF) I-dependent differentiation. We have investigated the domains of the IGF-IR required for differentiation of H19-7 cells. The tyrosine 950 residue and serines 1280-1283 in the COOH terminus of the receptor are required for IGF-I-induced differentiation at 39 degrees C, although they are dispensable for IGF-I-mediated growth at 34 degrees C. Both domains have to be mutated to inactivate the differentiating function. The inability of these mutant receptors to induce differentiation correlates with mitogen-activated protein kinase activation. In contrast, inhibitors of phosphatidylinositol 3'-kinase have no effect on IGF-I-mediated differentiation of H19-7 cells, although they do inhibit the mitogenic response.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Vesicular...,
http://linkedlifedata.com/resource/pubmed/chemical/IRS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin Receptor Substrate Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Irs1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Irs1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 1,
http://linkedlifedata.com/resource/pubmed/chemical/SHC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Shc Signaling Adaptor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Shc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Shc1 protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2263-72
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10786694-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10786694-Adaptor Proteins, Vesicular Transport,
pubmed-meshheading:10786694-Amino Acid Substitution,
pubmed-meshheading:10786694-Animals,
pubmed-meshheading:10786694-Cell Differentiation,
pubmed-meshheading:10786694-Cell Division,
pubmed-meshheading:10786694-Cell Line,
pubmed-meshheading:10786694-Enzyme Activation,
pubmed-meshheading:10786694-Fibroblasts,
pubmed-meshheading:10786694-Hippocampus,
pubmed-meshheading:10786694-Humans,
pubmed-meshheading:10786694-Insulin Receptor Substrate Proteins,
pubmed-meshheading:10786694-Insulin-Like Growth Factor I,
pubmed-meshheading:10786694-MAP Kinase Signaling System,
pubmed-meshheading:10786694-Mice,
pubmed-meshheading:10786694-Mitogen-Activated Protein Kinase Kinases,
pubmed-meshheading:10786694-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10786694-Mutation,
pubmed-meshheading:10786694-Neurons,
pubmed-meshheading:10786694-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:10786694-Phosphoproteins,
pubmed-meshheading:10786694-Phosphorylation,
pubmed-meshheading:10786694-Proteins,
pubmed-meshheading:10786694-Rats,
pubmed-meshheading:10786694-Receptor, IGF Type 1,
pubmed-meshheading:10786694-Shc Signaling Adaptor Proteins,
pubmed-meshheading:10786694-Temperature
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pubmed:year |
2000
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pubmed:articleTitle |
Insulin-like growth factor I receptor signaling in differentiation of neuronal H19-7 cells.
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pubmed:affiliation |
Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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