Source:http://linkedlifedata.com/resource/pubmed/id/10785398
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
9
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pubmed:dateCreated |
2000-6-15
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pubmed:databankReference | |
pubmed:abstractText |
Leaves of the monocotyledonous plant Polygonatum multiflorum L. (Solomon's seal) contain besides a monocot mannose-binding lectin two galactose/N-acetylgalactosamine (Gal/GalNAc)-binding type 2 ribosome-inactivating proteins (RIPs). Both RIPs were purified using a combination of classical protein purification techniques and affinity chromatography. Although both RIPs consist of protomers of 65 kDa, the P. multiflorum RIP monomer (PMRIPm) occurs as a monomer of approximately 60 kDa, whereas the tetramer (PMRIPt) is a tetramer of 240 kDa. Both RIPs exhibit similar RNA N-glycosidase activity but differ in their specific agglutination activity and carbohydrate-binding specificity, PMRIPt being a GalNAc-specific lectin whereas PMRIPm is Gal/GalNAc-specific. Toxicity tests indicated that both Polygonatum RIPs exhibit a very low cytotoxicity towards human and animal cells. Analysis of the genomic clones encoding both RIPs revealed a high degree of sequence similarity to other type 2 RIPs. Molecular modelling confirmed that both Polygonatum RIPs have a similar structure to ricin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-2956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
267
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2746-59
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pubmed:dateRevised |
2007-7-23
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pubmed:meshHeading |
pubmed-meshheading:10785398-Amino Acid Sequence,
pubmed-meshheading:10785398-Cloning, Molecular,
pubmed-meshheading:10785398-Cotyledon,
pubmed-meshheading:10785398-DNA, Complementary,
pubmed-meshheading:10785398-Lectins,
pubmed-meshheading:10785398-Liliaceae,
pubmed-meshheading:10785398-Models, Molecular,
pubmed-meshheading:10785398-Molecular Sequence Data,
pubmed-meshheading:10785398-Phylogeny,
pubmed-meshheading:10785398-Plant Lectins,
pubmed-meshheading:10785398-Plant Proteins,
pubmed-meshheading:10785398-Protein Conformation,
pubmed-meshheading:10785398-Sequence Homology, Amino Acid
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pubmed:year |
2000
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pubmed:articleTitle |
Characterization and molecular cloning of two different type 2 ribosome-inactivating proteins from the monocotyledonous plant Polygonatum multiflorum.
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pubmed:affiliation |
Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium. Els.VanDamme@agr.kuleuven.ac.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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