rdf:type |
|
lifeskim:mentions |
umls-concept:C0030685,
umls-concept:C0033497,
umls-concept:C0205102,
umls-concept:C0205314,
umls-concept:C0332283,
umls-concept:C0391871,
umls-concept:C0598829,
umls-concept:C0679622,
umls-concept:C0680255,
umls-concept:C1280500,
umls-concept:C1283071,
umls-concept:C1550548,
umls-concept:C1555714,
umls-concept:C1705654,
umls-concept:C1879547,
umls-concept:C1963578
|
pubmed:issue |
4
|
pubmed:dateCreated |
2000-7-14
|
pubmed:abstractText |
The effect of propranolol on Ca(2+) signalling in Madin Darby canine kidney (MDCK) cells was investigated by using fura-2 as a Ca(2+) probe. Propranolol increased cytosolic free Ca(2+) levels ([Ca(2+)](i)) in a concentration-dependent manner between 0.1 and 1 mM. The response was partly inhibited by external Ca(2+) removal. In Ca(2+)-free medium pretreatment with 0.2 mM propranolol partly inhibited the [Ca(2+)](i) rise induced by 1 microM thapsigargin, an inhibitor of the endoplasmic reticulum Ca(2+) pump; but pretreatment with thapsigargin abolished propranolol-induced Ca(2+) release. Addition of 3 mM Ca(2+) induced a [Ca(2+)](i) rise after pretreatment with 0.2 mM propranolol in Ca(2+)-free medium. Propranolol (0.2 mM) inhibited 25% of thapsigargin-induced capacitative Ca(2+) entry. Suppression of 1,4,5-trisphosphate (IP(3)) formation by 2 microM U73122, a phospholipase C inhibitor, did not alter 0.2 mM propranolol-induced internal Ca(2+) release. Propranolol (1 mM) also increased [Ca(2+)](i) in human neutrophils. Collectively, we have found that 0.2 mM propranolol increased [Ca(2+)](i) in MDCK cells by releasing Ca(2+) from thapsigargin-sensitive Ca(2+) stores in an IP(3)-independent manner, followed by Ca(2+) influx from external space. Independently, propranolol was able to inhibit thapsigargin-induced capacitative Ca(2+) entry.
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(6-((3-methoxyestra-1,3,5(10)-trie...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Estrenes,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Propranolol,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrrolidinones,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
|
pubmed:issn |
0898-6568
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
12
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
265-9
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10781934-Animals,
pubmed-meshheading:10781934-Biological Transport,
pubmed-meshheading:10781934-Calcium,
pubmed-meshheading:10781934-Calcium-Transporting ATPases,
pubmed-meshheading:10781934-Cell Line,
pubmed-meshheading:10781934-Cytosol,
pubmed-meshheading:10781934-Dogs,
pubmed-meshheading:10781934-Estrenes,
pubmed-meshheading:10781934-Humans,
pubmed-meshheading:10781934-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:10781934-Kidney,
pubmed-meshheading:10781934-Kinetics,
pubmed-meshheading:10781934-Neutrophils,
pubmed-meshheading:10781934-Phosphodiesterase Inhibitors,
pubmed-meshheading:10781934-Propranolol,
pubmed-meshheading:10781934-Pyrrolidinones,
pubmed-meshheading:10781934-Signal Transduction,
pubmed-meshheading:10781934-Thapsigargin
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pubmed:year |
2000
|
pubmed:articleTitle |
Novel effects of propranolol. Release of internal Ca(2+) followed by activation of capacitative Ca(2+) entry in Madin Darby canine kidney cells.
|
pubmed:affiliation |
Department of Medical Education and Research, Veterans General Hospital-Kaohsiung, Taiwan. crjan@isca.vghks.gov.tw
|
pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|