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rdf:type |
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|
lifeskim:mentions |
|
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pubmed:issue |
3
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pubmed:dateCreated |
2000-5-25
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|
pubmed:abstractText |
Mycoplasma hominis was transformed by electroporation with plasmid pAM120 containing the transposon Tn916 that carried the tetM gene responsible for the resistance to tetracycline. The frequency of transformation was 10(-7)-10(-8) colony-forming units (CFU) per 10 micrograms of plasmid DNA. The PCR analysis of transformed DNA confirmed the transposon integration into the mycoplasma genome.
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pubmed:language |
rus
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0016-6758
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
309-13
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10779903-Base Sequence,
pubmed-meshheading:10779903-DNA, Bacterial,
pubmed-meshheading:10779903-DNA Primers,
pubmed-meshheading:10779903-DNA Transposable Elements,
pubmed-meshheading:10779903-Electroporation,
pubmed-meshheading:10779903-Genome, Bacterial,
pubmed-meshheading:10779903-Mycoplasma hominis,
pubmed-meshheading:10779903-Plasmids,
pubmed-meshheading:10779903-Polymerase Chain Reaction,
pubmed-meshheading:10779903-RNA, Ribosomal, 16S,
pubmed-meshheading:10779903-Tetracycline Resistance,
pubmed-meshheading:10779903-Transformation, Genetic
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pubmed:year |
2000
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pubmed:articleTitle |
[Transformation of Mycoplasma hominis by plasmid pAM120 using electroporation].
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pubmed:affiliation |
Research Institute of Physicochemical Medicine, Moscow, Russia.
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pubmed:publicationType |
Journal Article,
English Abstract
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