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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
26
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pubmed:dateCreated |
2000-8-10
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pubmed:databankReference |
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pubmed:abstractText |
Gene targeting studies have demonstrated that the zinc finger transcription factor GATA-6 lies upstream in a transcriptional cascade that controls differentiation of the visceral endoderm. To understand the function of GATA-6 in the visceral endoderm and to identify genes regulated by GATA-6 in this tissue, subtractive hybridization was performed using template cDNAs derived from differentiated wild-type embryonic stem (ES) cells and GATA-6(-/-) ES cells, respectively. These analyses revealed that the gene encoding Dab2, a mitogen-responsive phosphoprotein, is differentially expressed in wild-type and GATA-6-deficient ES cells. Consistent with these findings, Dab2 is expressed in the visceral endoderm of wild-type embryos but not in the visceral endoderm of GATA-6-deficient embryos. Cotransfection experiments demonstrate that the human Dab2 promoter can be transactivated by forced expression of GATA-6 in NIH-3T3 cells. In contrast, forced expression of GATA-4 does not transactivate the human Dab2 promoter and Dab2 is expressed in the visceral endoderm of GATA-4 null embryos. Surprisingly, the specificity of GATA-6-induced transactivation of the Dab2 promoter is not mediated through its zinc finger DNA-binding domain. Taken together, these data demonstrate that the mitogen-responsive phosphoprotein Dab2 is a downstream target of GATA-6 in the visceral endoderm. Moreover, these data demonstrate that molecular mechanisms have evolved that direct, and distinguish, the functional specificity of GATA family members when they are developmentally coexpressed.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Vesicular...,
http://linkedlifedata.com/resource/pubmed/chemical/DAB2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GATA4 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Gata6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
19949-54
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10779506-3T3 Cells,
pubmed-meshheading:10779506-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10779506-Adaptor Proteins, Vesicular Transport,
pubmed-meshheading:10779506-Animals,
pubmed-meshheading:10779506-Base Sequence,
pubmed-meshheading:10779506-Cell Differentiation,
pubmed-meshheading:10779506-DNA, Complementary,
pubmed-meshheading:10779506-DNA-Binding Proteins,
pubmed-meshheading:10779506-Endoderm,
pubmed-meshheading:10779506-GATA4 Transcription Factor,
pubmed-meshheading:10779506-GATA6 Transcription Factor,
pubmed-meshheading:10779506-Gene Expression Regulation, Developmental,
pubmed-meshheading:10779506-Genes, Tumor Suppressor,
pubmed-meshheading:10779506-Humans,
pubmed-meshheading:10779506-In Situ Hybridization,
pubmed-meshheading:10779506-Mice,
pubmed-meshheading:10779506-Molecular Sequence Data,
pubmed-meshheading:10779506-Phosphoproteins,
pubmed-meshheading:10779506-Plasmids,
pubmed-meshheading:10779506-Promoter Regions, Genetic,
pubmed-meshheading:10779506-Protein Structure, Tertiary,
pubmed-meshheading:10779506-Proteins,
pubmed-meshheading:10779506-Recombinant Fusion Proteins,
pubmed-meshheading:10779506-Stem Cells,
pubmed-meshheading:10779506-Transcription, Genetic,
pubmed-meshheading:10779506-Transcription Factors,
pubmed-meshheading:10779506-Transcriptional Activation
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pubmed:year |
2000
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pubmed:articleTitle |
The gene encoding the mitogen-responsive phosphoprotein Dab2 is differentially regulated by GATA-6 and GATA-4 in the visceral endoderm.
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pubmed:affiliation |
Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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