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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2000-5-23
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pubmed:abstractText |
We examined the effects of retinoids on the human mast cell development using a serum-deprived culture system. When 10-week cultured mast cells derived from CD34(+) cord blood cells were used as target cells, both all-trans retinoic acid (ATRA) and 9-cis RA inhibited the progeny generation under stimulation with stem cell factor (SCF) in a dose-dependent manner (the number of progeny grown by SCF plus RA at 10(-7) mol/L was one tenth of the value obtained by SCF alone). The early steps in mast cell development appear to be less sensitive to RA according to the single CD34(+)c-kit(+) cord blood cell culture study. The optimal concentration of RAs also reduced the histamine concentration in the cultured mast cells (3.00 +/- 0.47 pg per cell in SCF alone, 1.44 +/- 0.18 pg per cell in SCF+ATRA, and 1.41 +/- 0.10 pg per cell in SCF+9-cis RA). RT-PCR analyses showed the expression of RARalpha, RARbeta, RXRalpha, and RXRbeta messenger ribonucleic acid (mRNA) in 10-week cultured mast cells. The addition of an RAR-selective agonist at 10(-10) mol/L to 10(-7) mol/L decreased the number of mast cells grown in SCF, whereas an RXR-selective agonist at up to 10(-8) mol/L was inactive. Among RAR subtype selective retinoids used at 10(-9) mol/L to 10(-7) mol/L, only the RARalpha agonist was equivalent to ATRA at 10(-7) mol/L in its ability to inhibit mast cell growth. Conversely, the addition of excess concentrations of a RARalpha antagonist profoundly counteracted the retinoid-mediated suppressive effects. These results suggest that RA inhibits SCF-dependent differentiation of human mast cell progenitors through a specific receptor. (Blood. 2000;95:2821-2828)
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoid X Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/alitretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/retinoic acid receptor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/retinoic acid receptor beta
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-4971
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pubmed:author |
pubmed-author:HiguchiTT,
pubmed-author:IshidaSS,
pubmed-author:ItoSS,
pubmed-author:KamijoTT,
pubmed-author:KawaSS,
pubmed-author:KinoshitaTT,
pubmed-author:KoikeKK,
pubmed-author:KomiyamaAA,
pubmed-author:KurokawaYY,
pubmed-author:MwamtemiH HHH,
pubmed-author:NakazawaYY,
pubmed-author:SakashitaKK,
pubmed-author:SawaiNN,
pubmed-author:ShioharaMM,
pubmed-author:TakeuchiKK,
pubmed-author:YamashitaTT
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2821-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10779427-Antigens, CD,
pubmed-meshheading:10779427-Antigens, CD34,
pubmed-meshheading:10779427-Cell Differentiation,
pubmed-meshheading:10779427-Cells, Cultured,
pubmed-meshheading:10779427-Culture Media, Serum-Free,
pubmed-meshheading:10779427-Fetal Blood,
pubmed-meshheading:10779427-Hematopoietic Stem Cells,
pubmed-meshheading:10779427-Histamine,
pubmed-meshheading:10779427-Humans,
pubmed-meshheading:10779427-Mast Cells,
pubmed-meshheading:10779427-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:10779427-Receptors, Retinoic Acid,
pubmed-meshheading:10779427-Retinoid X Receptors,
pubmed-meshheading:10779427-Stem Cell Factor,
pubmed-meshheading:10779427-Transcription, Genetic,
pubmed-meshheading:10779427-Transcription Factors,
pubmed-meshheading:10779427-Tretinoin
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pubmed:year |
2000
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pubmed:articleTitle |
Retinoic acid is a negative regulator for the differentiation of cord blood-derived human mast cell progenitors.
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pubmed:affiliation |
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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