Linked Life Data

10779159

Source:http://linkedlifedata.com/resource/pubmed/id/10779159

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-6-30
pubmed:abstractText
An important goal in cancer gene therapy is the development of novel targeted cytotoxic genes. The observation that transfection of a GaLV envelope glycoprotein lacking an R peptide into human cells results in considerable cell-cell fusion and subsequent cell death prompted us to explore the potential for using this fusogenic membrane glycoprotein (FMG) as a targeted cytotoxic gene. As proof of principle, we therefore displayed epidermal growth factor (EGF) on the N terminus of GaLV envelope glycoproteins both with and without an R peptide (GaLV R+ and GaLV R-). Transfection of the GaLVR+ envelope expression plasmids did not cause cell-cell fusion. The GaLV R+ envelopes were incorporated into retroviral vectors whose infectivity was investigated on EGF receptor-positive and -negative cells. The vector incorporating an N-terminally unmodified envelope was able to infect all human cell lines tested. Infectivity of the vector incorporating an envelope on which EGF was displayed was restricted on EGF receptor-positive cells (but not on EGF receptor-negative cells) and could be restored by protease cleavage of the displayed domain or competition with exogenous ligand. The cell-cell fusion capacity of the GaLV R- envelope glycoproteins (N-terminally unmodified and with N-terminal display of both EGF and insulin-like growth factor I [IGF-I]) was investigated by plasmid DNA transfection. While the N-terminally unmodified GaLV R- fused all human cell types tested, fusogenicity of GaLV R- on which EGF or IGF-I was displayed was considerably restricted on receptor-positive cells. "Reciprocal" competition experiments showed that fusogenicity could be restored by competition only with the relevant exogenous ligand. Thus the specificity of cell-cell fusion by a hyperfusogenic GaLV envelope glycoprotein can be regulated by N-terminal display of growth factor ligands. There is therefore significant potential for further development of the targeting of the cell-killing capability of this fusogenic viral glycoprotein by using strategies similar to those we have developed for the targeting of retroviral vectors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1043-0342
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
817-26
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10779159-Amino Acid Sequence, pubmed-meshheading:10779159-Animals, pubmed-meshheading:10779159-Cell Death, pubmed-meshheading:10779159-Cell Fusion, pubmed-meshheading:10779159-Epidermal Growth Factor, pubmed-meshheading:10779159-Gene Transfer Techniques, pubmed-meshheading:10779159-Genetic Vectors, pubmed-meshheading:10779159-Glycoproteins, pubmed-meshheading:10779159-Humans, pubmed-meshheading:10779159-Insulin-Like Growth Factor I, pubmed-meshheading:10779159-Leukemia Virus, Gibbon Ape, pubmed-meshheading:10779159-Ligands, pubmed-meshheading:10779159-Molecular Sequence Data, pubmed-meshheading:10779159-Oligopeptides, pubmed-meshheading:10779159-Receptor, Epidermal Growth Factor, pubmed-meshheading:10779159-Recombinant Fusion Proteins, pubmed-meshheading:10779159-Viral Envelope Proteins
pubmed:year
2000
pubmed:articleTitle
A hyperfusogenic gibbon ape leukemia envelope glycoprotein: targeting of a cytotoxic gene by ligand display.
pubmed:affiliation
Hematology and Molecular Medicine, Mayo Clinic, Rochester, MN 55902, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't