Linked Life Data

10778940

Source:http://linkedlifedata.com/resource/pubmed/id/10778940

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-8-1
pubmed:abstractText
A fully automated method including microbore liquid chromatography and column switching was developed for the analysis of biphenyldimethyl dicarboxylate (DDB) from human plasma samples. After direct injection of plasma samples (100 microl) into the system, deproteinization and analyte fractionation occurred on a Capcell Pak MF Ph-1 column (20x4 mm I.D.) and the DDB fraction was transferred from the MF Ph-1 column to an intermediate column (35x2 mm I.D.) using 15% acetonitrile in phosphate buffer (50 mM, pH 7.0). The main separation was performed on a microbore C18 column (150x1.5 mm I.D.) using 45% acetonitrile in water. The method showed excellent sensitivity (detection limit of 5 ng/ml) and good precision (CV.< or =3.0%), and shortened total analysis time (20 min). In the concentration range of 5-200 ng/ml, the mean recovery was 90.7+/-1.8% and the response was linear (r2> or =0.999).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1387-2273
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
738
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
175-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Rapid microbore liquid chromatographic analysis of biphenyldimethyl dicarboxylate in human plasma with on-line column switching.
pubmed:affiliation
College of Pharmacy, Medical Resources Research Center, Wonkwang University, Iksan, South Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't