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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2000-6-26
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pubmed:abstractText |
InsP(3)-evoked elementary Ca(2+) release events have been postulated to play a role in providing the building blocks of larger Ca(2+) signals. In pancreatic acinar cells, low concentrations of acetylcholine or the injection of low concentrations of InsP(3) elicit a train of spatially localized Ca(2+) spikes. In this study we have quantified these responses and compared the Ca(2+) signals to the elementary events shown in Xenopus oocytes. The results demonstrate, at the same concentrations of InsP(3), Ca(2+) signals consisting of one population of small transient Ca(2+) release events and a second distinct population of larger Ca(2+) spikes. The signal mass amplitudes of both types of events are within the range of amplitudes for the elementary events in Xenopus oocytes. However, the bimodal Ca(2+) distribution of Ca(2+) responses we observe is not consistent with the continuum of event sizes seen in Xenopus. We conclude that the two types of InsP(3)-dependent events in acinar cells are both elementary Ca(2+) signals, which are independent of one another. Our data indicate a complexity to the organization of the Ca(2+) release apparatus in acinar cells, which might result from the presence of multiple InsP(3) receptor isoforms, and is likely to be important in the physiology of these cells.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-10064596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-10427093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-10498675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-10517811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-10531009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-1375633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-1380978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-2335224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-7514529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-7570021,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-7664312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-7738847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-7744807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-7961809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-8395347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-8395348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-8605895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9029145,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9065779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9080360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9080361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9083036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9154905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9188472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9363945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9422695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9515967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9535884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9547382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9582075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9624056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9675184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9765231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9817204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10777728-9838027
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2298-306
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10777728-Animals,
pubmed-meshheading:10777728-Biophysical Phenomena,
pubmed-meshheading:10777728-Biophysics,
pubmed-meshheading:10777728-Calcium Channels,
pubmed-meshheading:10777728-Calcium Signaling,
pubmed-meshheading:10777728-Female,
pubmed-meshheading:10777728-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:10777728-Inositol 1,4,5-Trisphosphate Receptors,
pubmed-meshheading:10777728-Male,
pubmed-meshheading:10777728-Mice,
pubmed-meshheading:10777728-Microscopy, Fluorescence,
pubmed-meshheading:10777728-Oocytes,
pubmed-meshheading:10777728-Pancreas,
pubmed-meshheading:10777728-Patch-Clamp Techniques,
pubmed-meshheading:10777728-Protein Isoforms,
pubmed-meshheading:10777728-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:10777728-Xenopus
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pubmed:year |
2000
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pubmed:articleTitle |
A bimodal pattern of InsP(3)-evoked elementary Ca(2+) signals in pancreatic acinar cells.
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pubmed:affiliation |
Biomedical Imaging Group, Department of Physiology, University of Massachusetts Medical School, Worcester, Massachusetts 01650 USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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