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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
2000-6-2
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pubmed:abstractText |
mRNA transcribed from the mouse KC chemokine gene accumulated to significantly higher levels in multiple cell types after treatment with interleukin 1alpha (IL-1alpha) as compared with tumor necrosis factor-alpha (TNFalpha). Although TNFalpha and IL-1alpha both signaled the activation of nuclear factor kappaB and enhanced transcription of the KC gene with equal potency, only IL-1alpha treatment resulted in stabilization of KC mRNA. Nucleotide sequences that confer sensitivity for IL-1alpha-mediated mRNA stabilization were identified within the 5'- and 3'-untranslated regions (UTRs) of KC mRNA using transient transfection of chimeric plasmids containing specific portions of KC mRNA linked to the chloramphenicol acetyltransferase (CAT) gene. When plasmids containing either the 3'- or 5'-UTR of KC mRNA were used, the half-life of CAT mRNA was unaltered either in untreated or IL-1alpha-stimulated cells. In contrast, CAT mRNA transcribed from plasmids that contained both the 5'- and 3'-UTRs of the KC mRNA decayed more rapidly than control CAT mRNA, and this enhanced decay was prevented in cells treated with IL-1alpha. A cluster of four overlapping AUUUA motifs within the 3'-UTR was required, whereas the 5'-UTR region exhibited orientation dependence. These findings indicate that cooperative function of the two nucleotide sequences involves a distinct signaling pathway used by IL-1alpha but not TNFalpha.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CXCL1,
http://linkedlifedata.com/resource/pubmed/chemical/Chemokines, CXC,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Cxcl1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleic Acid Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12987-93
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10777600-3' Untranslated Regions,
pubmed-meshheading:10777600-3T3 Cells,
pubmed-meshheading:10777600-5' Untranslated Regions,
pubmed-meshheading:10777600-Animals,
pubmed-meshheading:10777600-Cell Line,
pubmed-meshheading:10777600-Chemokine CXCL1,
pubmed-meshheading:10777600-Chemokines, CXC,
pubmed-meshheading:10777600-Chemotactic Factors,
pubmed-meshheading:10777600-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:10777600-Dactinomycin,
pubmed-meshheading:10777600-Growth Substances,
pubmed-meshheading:10777600-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:10777600-Interleukin-1,
pubmed-meshheading:10777600-Mice,
pubmed-meshheading:10777600-Mice, Inbred BALB C,
pubmed-meshheading:10777600-Models, Genetic,
pubmed-meshheading:10777600-NF-kappa B,
pubmed-meshheading:10777600-Nucleic Acid Synthesis Inhibitors,
pubmed-meshheading:10777600-Promoter Regions, Genetic,
pubmed-meshheading:10777600-RNA, Messenger,
pubmed-meshheading:10777600-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:10777600-Time Factors,
pubmed-meshheading:10777600-Transcription, Genetic,
pubmed-meshheading:10777600-Transfection,
pubmed-meshheading:10777600-Tumor Necrosis Factor-alpha
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pubmed:year |
2000
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pubmed:articleTitle |
Interleukin-1-mediated stabilization of mouse KC mRNA depends on sequences in both 5'- and 3'-untranslated regions.
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pubmed:affiliation |
Department of Immunology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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