Source:http://linkedlifedata.com/resource/pubmed/id/10775035
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2000-6-9
|
| pubmed:abstractText |
The c-Raf-1 kinase is a downstream effector of Ras signaling. Both proteins are highly oncogenic when they are mutationally activated, but only the Ras GTPase is frequently mutated in naturally occurring tumors. Although the c-Raf-1 protein was found to be amplified in different lung cancer cell lines, overexpression of the wild-type c-Raf-1 protein was shown to be insufficient to transform cultured cells. Here we have addressed the question of whether overexpression of the wild-type c-Raf-1 kinase can induce lung cancer in mice. We show that lung-targeted expression of oncogenically activated or wild-type c-Raf-1 proteins induces morphologically indistinguishable lung adenomas in transgenic mice. Compared with mice transgenic for the activated c-Raf-1-BxB, tumor development is delayed and occurs at a lower incidence in wild-type c-Raf-1 transgenic mice. Our studies show that the c-Raf-1 expression level is a critical parameter in tumor development and should be analyzed in more detail to evaluate its potential in the induction of cancer.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
1044-9523
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
185-90
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:10775035-Adenoma,
pubmed-meshheading:10775035-Animals,
pubmed-meshheading:10775035-Female,
pubmed-meshheading:10775035-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10775035-Humans,
pubmed-meshheading:10775035-Immunohistochemistry,
pubmed-meshheading:10775035-Lung,
pubmed-meshheading:10775035-Lung Neoplasms,
pubmed-meshheading:10775035-Male,
pubmed-meshheading:10775035-Mice,
pubmed-meshheading:10775035-Mice, Inbred C57BL,
pubmed-meshheading:10775035-Mice, Inbred DBA,
pubmed-meshheading:10775035-Mice, Transgenic,
pubmed-meshheading:10775035-Promoter Regions, Genetic,
pubmed-meshheading:10775035-Proteolipids,
pubmed-meshheading:10775035-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:10775035-Pulmonary Surfactants,
pubmed-meshheading:10775035-Recombinant Fusion Proteins
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Lung-targeted expression of the c-Raf-1 kinase in transgenic mice exposes a novel oncogenic character of the wild-type protein.
|
| pubmed:affiliation |
Institut für medizinische Strahlenkunde und Zellforschung, Universität Würzburg, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|