Source:http://linkedlifedata.com/resource/pubmed/id/10773410
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2000-6-2
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| pubmed:abstractText |
Transduction of the human interleukin-2 (IL-2) gene into tumor cells was carried out in order to develop a new immunotherapy for advanced head and neck carcinomas with a poor outcome. We transduced the IL-2 gene into KB cells, a head and neck squamous cell carcinoma cell line, using a defective herpes simplex viral (HSV) amplicon vector as a gene transfer vehicle. A high level of IL-2 was secreted by IL-2 gene-transduced KB cells (KB/IL-2). The IL-2 producibility of irradiated KB/IL-2 cells was almost the same as that of non-irradiated cells. In the tumor establishment model in nude mice, IL-2 and interferon-gamma (IFN-gamma) at high concentrations were detected in the sera of mice transplanted with KB/IL-2 cells. The spleen cells of nude mice transplanted with KB/IL-2 cells exhibited high cytotoxic activity compared to those from mice transplanted with KB cells and from untreated mice. Three of five mice transplanted with KB/IL-2 cells rejected tumors. In the treatment of established tumors, therapeutic effects due to irradiated KB/IL-2 were dose-dependent. The suppressive effects on tumor growth were blocked by anti-asialo GM1, anti-human IL-2 and anti-IFN-gamma antibodies. Immunohistochemical observation revealed the presence of asialo GM1(+) cells among the KB/IL-2 cells in tumors transplanted into nude mice.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0304-3835
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
152
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
175-85
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10773410-Animals,
pubmed-meshheading:10773410-Cancer Vaccines,
pubmed-meshheading:10773410-Carcinoma, Squamous Cell,
pubmed-meshheading:10773410-Cercopithecus aethiops,
pubmed-meshheading:10773410-Female,
pubmed-meshheading:10773410-Gene Therapy,
pubmed-meshheading:10773410-Genetic Vectors,
pubmed-meshheading:10773410-Head and Neck Neoplasms,
pubmed-meshheading:10773410-Humans,
pubmed-meshheading:10773410-Immunohistochemistry,
pubmed-meshheading:10773410-Interferon-gamma,
pubmed-meshheading:10773410-Interleukin-2,
pubmed-meshheading:10773410-Mice,
pubmed-meshheading:10773410-Mice, Inbred BALB C,
pubmed-meshheading:10773410-Mice, Nude,
pubmed-meshheading:10773410-Neoplasm Transplantation,
pubmed-meshheading:10773410-Plasmids,
pubmed-meshheading:10773410-Simplexvirus,
pubmed-meshheading:10773410-Spleen,
pubmed-meshheading:10773410-Time Factors,
pubmed-meshheading:10773410-Tumor Cells, Cultured,
pubmed-meshheading:10773410-Vero Cells
|
| pubmed:year |
2000
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| pubmed:articleTitle |
Therapeutic efficiency of IL-2 gene transduced tumor vaccine for head and neck carcinoma.
|
| pubmed:affiliation |
Department of Otolaryngology, Yokohama City University School of Medicine, 3-9 Fukuura Kanazawa-ku, Yokohama, Japan. m_163@med.yokohama-cu.ac.jp
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| pubmed:publicationType |
Journal Article
|