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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-7-13
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pubmed:abstractText |
We have investigated the interaction of the SH2-containing protein tyrosine phosphatase-1 (SHP-1) and Jak2 in an erythropoietin (Epo)-dependent human leukemia cell line, UT-7/Epo, using reciprocal immunoprecipitation and immunoblotting. The Epo-induced kinetics and dose response on phosphorylated Jak2 in anti-SHP-1 precipitates of UT-7/Epo cell lysates were similar to those in direct anti-Jak2 precipitates, suggesting that Jak2 coprecipitated with SHP-1. Furthermore, immunoblotting with anti-Jak2 and anti-SHP-1 antibodies indicated that SHP-1 appeared to be constitutively associated with non-tyrosine-phosphorylated Jak2 in UT-7/Epo cells in the absence of Epo and without phosphorylation of the Epo receptor (EpoR). Competition studies with C-terminal SHP-1 and Jak2 peptides decreased the amounts of SHP-1 and Jak2 detected in immunoprecipitates supporting the specific coprecipitation of SHP-1 and Jak2. In the presence of a recombinant GST-fusion protein containing both the N-terminal and C-terminal SH2 domains of SHP-1, anti-GST precipitated the fusion protein but not cellular Jak2. These studies suggest that SHP-1 and Jak2 are constitutively associated in UT-7/EPO cells. The association is not dependent upon Epo and is not mediated via SHP-1 SH2 binding. Sequential double immunoprecipitation demonstrated that only a small portion of intracellular Jak2 and SHP-1 molecules are constitutively associated. This partial association pattern may allow a more flexible and diverse regulation of Jak2 and SHP-1 activities. Whether Jak2 and SHP-1 are directly associated with each other or are part of a larger complex needs further investigation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Intracellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/JAK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/PTPN11 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTPN6 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Phosphatase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/SH2 Domain-Containing Protein...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1079-9796
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15-24
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10772872-Antibodies,
pubmed-meshheading:10772872-Binding, Competitive,
pubmed-meshheading:10772872-Dose-Response Relationship, Drug,
pubmed-meshheading:10772872-Erythropoietin,
pubmed-meshheading:10772872-Humans,
pubmed-meshheading:10772872-Immunoblotting,
pubmed-meshheading:10772872-Intracellular Signaling Peptides and Proteins,
pubmed-meshheading:10772872-Janus Kinase 2,
pubmed-meshheading:10772872-Kinetics,
pubmed-meshheading:10772872-Peptides,
pubmed-meshheading:10772872-Phosphorylation,
pubmed-meshheading:10772872-Precipitin Tests,
pubmed-meshheading:10772872-Protein Binding,
pubmed-meshheading:10772872-Protein Phosphatase 1,
pubmed-meshheading:10772872-Protein Tyrosine Phosphatase, Non-Receptor Type 11,
pubmed-meshheading:10772872-Protein Tyrosine Phosphatase, Non-Receptor Type 6,
pubmed-meshheading:10772872-Protein Tyrosine Phosphatases,
pubmed-meshheading:10772872-Protein-Tyrosine Kinases,
pubmed-meshheading:10772872-Proto-Oncogene Proteins,
pubmed-meshheading:10772872-Receptors, Erythropoietin,
pubmed-meshheading:10772872-SH2 Domain-Containing Protein Tyrosine Phosphatases,
pubmed-meshheading:10772872-Signal Transduction,
pubmed-meshheading:10772872-Tumor Cells, Cultured,
pubmed-meshheading:10772872-src Homology Domains
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pubmed:year |
2000
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pubmed:articleTitle |
SH2-Containing protein tyrosine phosphatase-1 (SHP-1) association with Jak2 in UT-7/Epo cells.
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pubmed:affiliation |
Department of Molecular Virology and Host Defense, SmithKline Beecham Pharmaceuticals, Collegeville, Pennsylvania, USA.
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pubmed:publicationType |
Journal Article
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