Source:http://linkedlifedata.com/resource/pubmed/id/10772531
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2000-6-7
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| pubmed:abstractText |
Human immunodeficiency virus type 1 (HIV-1) has been found in the vitreous of persons with AIDS. Here we investigated the susceptibility of human retinal pigment epithelial (RPE) cells to HIV-1 infection in culture and the effects of HIV-1 on the phagocytic function of the RPE. We found that 10 of 11 populations of RPE cells isolated from different fetal or adult eyes were susceptible to low-level replication of HIV-1/NL4-3 as determined by the detection of viral DNA and spliced viral RNA encoding envelope. HIV-1 infection was not inhibited by recombinant soluble CD4, suggesting that CD4 is not required for virus entry into RPE cells. RPE cells fused with target cells constitutively expressing HIV-1 envelope glycoproteins, indicating that HIV-1 enters cells by receptor-mediated fusion. Exposure to HIV-1 or recombinant gp120 caused a two- to four-fold increase in the binding and uptake of isolated rod outer segments by RPE cells. These findings introduce a new cell target of HIV-1 replication in the eye and indicate that RPE cells function aberrantly when exposed to HIV-1 or its envelope glycoprotein.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/HIV Envelope Protein gp120,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0889-2229
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
453-63
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10772531-Adult,
pubmed-meshheading:10772531-Animals,
pubmed-meshheading:10772531-Antigens, CD4,
pubmed-meshheading:10772531-CHO Cells,
pubmed-meshheading:10772531-Cell Fusion,
pubmed-meshheading:10772531-Cells, Cultured,
pubmed-meshheading:10772531-Cricetinae,
pubmed-meshheading:10772531-DNA, Viral,
pubmed-meshheading:10772531-Epithelial Cells,
pubmed-meshheading:10772531-Fetus,
pubmed-meshheading:10772531-HIV Envelope Protein gp120,
pubmed-meshheading:10772531-HIV-1,
pubmed-meshheading:10772531-Humans,
pubmed-meshheading:10772531-Phagocytosis,
pubmed-meshheading:10772531-Pigment Epithelium of Eye,
pubmed-meshheading:10772531-Polymerase Chain Reaction,
pubmed-meshheading:10772531-RNA, Viral,
pubmed-meshheading:10772531-Recombinant Proteins,
pubmed-meshheading:10772531-Rod Cell Outer Segment,
pubmed-meshheading:10772531-Virus Replication
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
Human immunodeficiency virus type 1 can infect human retinal pigment epithelial cells in culture and alter the ability of the cells to phagocytose rod outer segment membranes.
|
| pubmed:affiliation |
Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital Center, Columbia University, New York, New York 10019, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|