Source:http://linkedlifedata.com/resource/pubmed/id/10771523
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2000-8-15
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| pubmed:abstractText |
During development, mRNA for matrix metalloproteinase-13 (MMP-13) is found associated with cartilage undergoing hypertrophy, suggesting that this collagenase plays a role in cell enlargement and/or cartilage calcification. Using chondrocytes from prehypertrophic cartilage of chick embryo sternae, we have examined the relationship between MMP-13 expression and the transition to hypertrophy. When hypertrophy was induced by serum-free culture with ascorbate and bone morphogenetic protein-2 (BMP-2), MMP-13 mRNA levels paralleled those for type X collagen. Chondrocytes from the caudal, nonhypertrophying portion of chick sternae expressed neither type X collagen nor MMP-13, confirming that MMP-13 mRNA is a marker for hypertrophy. Zymography with conditioned medium yielded a proteinase band at 59 kDa, which was absent in nonhypertrophic chondrocytes. A polyclonal antibody raised against chick MMP-13 reacted with the 59-kDa protein, confirming that it is MMP-13. Although mRNA for MMP-13 peaked at days 4-5 of culture, only low levels of MMP-13 activity were present, and the activity increased gradually in parallel with later increases in MMP-2. These results suggest that MMP-13 is activated by MMP-2 during chondrocyte maturation, and that the combination of both proteinases is required to prepare cartilage matrix for subsequent calcification, before endochondral ossification.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Collagenases,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 13,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0730-2312
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
77
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
678-93
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10771523-Animals,
pubmed-meshheading:10771523-Biological Markers,
pubmed-meshheading:10771523-Blotting, Northern,
pubmed-meshheading:10771523-Blotting, Western,
pubmed-meshheading:10771523-Cartilage,
pubmed-meshheading:10771523-Chick Embryo,
pubmed-meshheading:10771523-Chondrocytes,
pubmed-meshheading:10771523-Collagen,
pubmed-meshheading:10771523-Collagenases,
pubmed-meshheading:10771523-Culture Media, Serum-Free,
pubmed-meshheading:10771523-DNA, Complementary,
pubmed-meshheading:10771523-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10771523-Hypertrophy,
pubmed-meshheading:10771523-Immunohistochemistry,
pubmed-meshheading:10771523-In Situ Hybridization,
pubmed-meshheading:10771523-Matrix Metalloproteinase 13,
pubmed-meshheading:10771523-Matrix Metalloproteinase 2,
pubmed-meshheading:10771523-Models, Biological,
pubmed-meshheading:10771523-RNA, Messenger,
pubmed-meshheading:10771523-Sternum,
pubmed-meshheading:10771523-Time Factors
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| pubmed:year |
2000
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| pubmed:articleTitle |
MMP-13 is induced during chondrocyte hypertrophy.
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| pubmed:affiliation |
Department of Biochemistry, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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