Linked Life Data

10771396

Source:http://linkedlifedata.com/resource/pubmed/id/10771396

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2000-8-10
pubmed:abstractText
In our initial investigation of functionality of platelets freeze-dried after stabilization with 1.8% paraformaldehyde, we found that the rehydrated cells were morphologically intact and retained adhesive and procoagulant properties. Further testing of fixed, washed freeze-dried platelets has demonstrated the physiologic nature of their adhesion in vitro and their hemostatic efficacy in vivo in correcting the bleeding time in thrombocytopenic animal models. Binding studies with monoclonal antibodies and radiolabelled ligands indicate an intact GpIb vonWillebrands factor receptor as on fresh platelets, but a somewhat attenuated GpIIbIIIa fibrinogen receptor. Repeated infusion of canine lyophilized platelet preparations in a single recipient over several months has shown no incipient cytopenia upon infusion of new doses nor accelerated clearance of platelets. These findings suggest minimal risk of systemic thrombosis or severe immunogenic reaction and support the notion of approaching clinical trials as soon as possible.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
T
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0955-3886
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
99-105
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
Lyophilized platelets: continued development.
pubmed:affiliation
Department of Pathology & Laboratory Medicine, East Carolina University, Greenville, NC, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.