| pubmed-article:10770806 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C0005821 | lld:lifeskim |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C1171362 | lld:lifeskim |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C1419941 | lld:lifeskim |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C1515670 | lld:lifeskim |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:10770806 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:10770806 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:10770806 | pubmed:dateCreated | 2000-5-9 | lld:pubmed |
| pubmed-article:10770806 | pubmed:abstractText | The platelet plays a pivotal role in maintaining vascular integrity. In a manner similar to leukocytes, platelets interact with selectins expressed on activated endothelium. P-selectin glycoprotein ligand 1 (PSGL-1) is the main P-selectin ligand expressed on leukocytes. Searching for platelet ligand(s), we used a P-selectin-immunoglobulin G (IgG) chimera to affinity purify surface-biotinylated proteins from platelet lysates. P-selectin-bound ligands were eluted with ethylenediaminetetraacetic acid. An approximately 210-kD biotinylated protein was isolated from both human neutrophil and platelet preparations. A band of the same size was also immunopurified from human platelets using a monoclonal anti-human PSGL-1 antibody and could be blotted with P-selectin-IgG. Under reducing conditions, both the predicted PSGL-1 approximately 210-kD dimer and the approximately 120-kD monomer were isolated from platelets. Comparative immunoelectron microscopy and Western blotting experiments suggested that platelet PSGL-1 expression is 25-100-fold lower than that of leukocytes. However, patients with chronic idiopathic thrombocytopenic purpura who harbor predominantly young platelets displayed greater expression, indicating that PSGL-1 expression may be decreased during platelet aging. By flow cytometry, thrombin-activated platelets from normal individuals exhibited greater expression than those unstimulated. An inhibitory anti-PSGL-1 antibody significantly reduced platelet rolling in mesenteric venules, as observed by intravital microscopy. Our results indicate that functional PSGL-1 is expressed on platelets, and suggest an additional mechanism by which selectins and their ligands participate in inflammatory and/or hemostatic responses. | lld:pubmed |
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| pubmed-article:10770806 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10770806 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10770806 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10770806 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10770806 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10770806 | pubmed:issn | 0022-1007 | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:WeissLL | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:HartwigJ HJH | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:KehrelBB | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:WagnerD DDD | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:VestweberDD | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:JuriMM | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:FrenetteP SPS | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:SubbaraoSS | lld:pubmed |
| pubmed-article:10770806 | pubmed:author | pubmed-author:DenisC VCV | lld:pubmed |
| pubmed-article:10770806 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10770806 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:10770806 | pubmed:volume | 191 | lld:pubmed |
| pubmed-article:10770806 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10770806 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10770806 | pubmed:pagination | 1413-22 | lld:pubmed |
| pubmed-article:10770806 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:10770806 | pubmed:meshHeading | pubmed-meshheading:10770806... | lld:pubmed |
| pubmed-article:10770806 | pubmed:meshHeading | pubmed-meshheading:10770806... | lld:pubmed |
| pubmed-article:10770806 | pubmed:meshHeading | pubmed-meshheading:10770806... | lld:pubmed |
| pubmed-article:10770806 | pubmed:meshHeading | pubmed-meshheading:10770806... | lld:pubmed |
| pubmed-article:10770806 | pubmed:meshHeading | pubmed-meshheading:10770806... | lld:pubmed |
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| pubmed-article:10770806 | pubmed:meshHeading | pubmed-meshheading:10770806... | lld:pubmed |
| pubmed-article:10770806 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10770806 | pubmed:articleTitle | P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo. | lld:pubmed |
