|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
2000-5-9
|
|
pubmed:abstractText |
The platelet plays a pivotal role in maintaining vascular integrity. In a manner similar to leukocytes, platelets interact with selectins expressed on activated endothelium. P-selectin glycoprotein ligand 1 (PSGL-1) is the main P-selectin ligand expressed on leukocytes. Searching for platelet ligand(s), we used a P-selectin-immunoglobulin G (IgG) chimera to affinity purify surface-biotinylated proteins from platelet lysates. P-selectin-bound ligands were eluted with ethylenediaminetetraacetic acid. An approximately 210-kD biotinylated protein was isolated from both human neutrophil and platelet preparations. A band of the same size was also immunopurified from human platelets using a monoclonal anti-human PSGL-1 antibody and could be blotted with P-selectin-IgG. Under reducing conditions, both the predicted PSGL-1 approximately 210-kD dimer and the approximately 120-kD monomer were isolated from platelets. Comparative immunoelectron microscopy and Western blotting experiments suggested that platelet PSGL-1 expression is 25-100-fold lower than that of leukocytes. However, patients with chronic idiopathic thrombocytopenic purpura who harbor predominantly young platelets displayed greater expression, indicating that PSGL-1 expression may be decreased during platelet aging. By flow cytometry, thrombin-activated platelets from normal individuals exhibited greater expression than those unstimulated. An inhibitory anti-PSGL-1 antibody significantly reduced platelet rolling in mesenteric venules, as observed by intravital microscopy. Our results indicate that functional PSGL-1 is expressed on platelets, and suggest an additional mechanism by which selectins and their ligands participate in inflammatory and/or hemostatic responses.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-10077522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-10348699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-10386598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-10499919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-10555146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-1704009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-1991165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7505206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7512971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7522621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7528665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7532110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7538108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7545173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7585949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7585950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7592904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7686912,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-7694385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-8541529,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-8565074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-8756756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-8874199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-8876231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-8896391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9053457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9150453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9242884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9292527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9414280,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9454762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9638971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9657750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9751682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9834252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10770806-9922371
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0022-1007
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
17
|
|
pubmed:volume |
191
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1413-22
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10770806-Animals,
pubmed-meshheading:10770806-Antibodies, Monoclonal,
pubmed-meshheading:10770806-Base Sequence,
pubmed-meshheading:10770806-Blood Platelets,
pubmed-meshheading:10770806-DNA Primers,
pubmed-meshheading:10770806-Endothelium, Vascular,
pubmed-meshheading:10770806-Gene Expression,
pubmed-meshheading:10770806-Humans,
pubmed-meshheading:10770806-Leukocytes,
pubmed-meshheading:10770806-Ligands,
pubmed-meshheading:10770806-Male,
pubmed-meshheading:10770806-Membrane Glycoproteins,
pubmed-meshheading:10770806-Mice,
pubmed-meshheading:10770806-Mice, Inbred C57BL,
pubmed-meshheading:10770806-Microscopy, Immunoelectron,
pubmed-meshheading:10770806-P-Selectin,
pubmed-meshheading:10770806-Platelet Activation,
pubmed-meshheading:10770806-RNA, Messenger
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo.
|
