Source:http://linkedlifedata.com/resource/pubmed/id/10769689
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1A
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| pubmed:dateCreated |
2000-5-11
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| pubmed:abstractText |
Differential expression of the desired gene product in the target tissue is central to the concept of gene therapy. One approach is to use a tissue-specific promoter to drive therapeutic genes, such as the p53 tumor suppressor gene. To determine the feasibility of tissue-specific gene therapy for prostate cancer using prostate specific antigen (PSA) promoter and/or enhancer, in this study, we developed a tissue specific expression vector using a PSA promoter and enhancer. Our results showed that the cloned PSA promoter actively drives gene expression in the PSA-producing prostate cancer cell line (LNCaP). However, barely any promoter activity was detected in the non-PSA producing prostate cancer cell lines (DU145, PC-3) or the non-prostate cell lines (HEK-293, SAOS-2). The wild-type p53 gene driven by this PSA-promoter efficiently suppressed the growth of LNCaP. Moreover, p53 driven by the PSA enhancer-promoter cassette more efficiently suppressed the growth of the PSA-producing prostate cancer cell line (LNCaP) in vitro. This suggest that we were able to manage the tissue specificity by PSA enhancer and promoter. Additionally, the juxtaposed enhancer-promoter cassette showed great enhancement of p53 expression and apoptosis in vitro. Taken together, these results show that PSA enhancer-promoter may be a potential tool for gene therapy for prostate cancer.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Prostate-Specific Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0250-7005
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
417-22
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10769689-Adenocarcinoma,
pubmed-meshheading:10769689-Antigens, Neoplasm,
pubmed-meshheading:10769689-Caspase 3,
pubmed-meshheading:10769689-Caspases,
pubmed-meshheading:10769689-Enhancer Elements, Genetic,
pubmed-meshheading:10769689-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:10769689-Gene Therapy,
pubmed-meshheading:10769689-Genes, p53,
pubmed-meshheading:10769689-Genetic Vectors,
pubmed-meshheading:10769689-Humans,
pubmed-meshheading:10769689-Male,
pubmed-meshheading:10769689-Organ Specificity,
pubmed-meshheading:10769689-Promoter Regions, Genetic,
pubmed-meshheading:10769689-Prostate-Specific Antigen,
pubmed-meshheading:10769689-Prostatic Neoplasms,
pubmed-meshheading:10769689-Recombinant Fusion Proteins,
pubmed-meshheading:10769689-Tumor Cells, Cultured,
pubmed-meshheading:10769689-Tumor Suppressor Protein p53
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| pubmed:articleTitle |
Development of a new plasmid vector with PSA-promoter and enhancer expressing tissue-specificity in prostate carcinoma cell lines.
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| pubmed:affiliation |
Department of Urology, Seoul National University College of Medicine, Korea. urology@plaza.snu.ac.kr
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| pubmed:publicationType |
Journal Article,
Comparative Study
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