| pubmed-article:10768928 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10768928 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:10768928 | lifeskim:mentions | umls-concept:C0001699 | lld:lifeskim |
| pubmed-article:10768928 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:10768928 | lifeskim:mentions | umls-concept:C0017258 | lld:lifeskim |
| pubmed-article:10768928 | lifeskim:mentions | umls-concept:C0699748 | lld:lifeskim |
| pubmed-article:10768928 | lifeskim:mentions | umls-concept:C1561491 | lld:lifeskim |
| pubmed-article:10768928 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:10768928 | pubmed:dateCreated | 2000-6-13 | lld:pubmed |
| pubmed-article:10768928 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10768928 | pubmed:abstractText | One representative recombinant clone encoding Klebsiella pneumoniae O5-antigen lipopolysaccharide (LPS) was found upon screening for serum resistance in a cosmid-based genomic library of K. pneumoniae KT769 (O5:K57) introduced into Escherichia coli DH5alpha. A total of eight open reading frames (wb(O5) gene cluster) were necessary to produce K. pneumoniae O5-antigen LPS in E. coli K-12. The enzymatic activities proposed for the wb(O5) gene cluster are in agreement with the activities proposed for the biosynthesis of K. pneumoniae O5-antigen LPS. Using the complete DNA sequence of the K. pneumoniae wb(O5) gene cluster, we obtained (by single or double recombination) genetically well-characterized mutants devoid only of this O5-antigen LPS. Finally, using these O5(-) mutants and the corresponding wild-type strains or complemented mutants with the wb(O5) gene cluster (O5(+) strains), we found that the presence of K. pneumoniae O5-antigen LPS is essential for some pathogenic features like serum resistance, adhesion to uroepithelial cells, and colonization (experimental infections) of the urinary tract in rats. | lld:pubmed |
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| pubmed-article:10768928 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10768928 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10768928 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:10768928 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10768928 | pubmed:month | May | lld:pubmed |
| pubmed-article:10768928 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:10768928 | pubmed:author | pubmed-author:TomásJ MJM | lld:pubmed |
| pubmed-article:10768928 | pubmed:author | pubmed-author:IzquierdoLL | lld:pubmed |
| pubmed-article:10768928 | pubmed:author | pubmed-author:MerinoSS | lld:pubmed |
| pubmed-article:10768928 | pubmed:author | pubmed-author:ReguéMM | lld:pubmed |
| pubmed-article:10768928 | pubmed:author | pubmed-author:NoguerasM MMM | lld:pubmed |
| pubmed-article:10768928 | pubmed:author | pubmed-author:AltarribaMM | lld:pubmed |
| pubmed-article:10768928 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10768928 | pubmed:volume | 68 | lld:pubmed |
| pubmed-article:10768928 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10768928 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10768928 | pubmed:pagination | 2435-40 | lld:pubmed |
| pubmed-article:10768928 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:10768928 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10768928 | pubmed:articleTitle | Cloning and sequencing of the Klebsiella pneumoniae O5 wb gene cluster and its role in pathogenesis. | lld:pubmed |
| pubmed-article:10768928 | pubmed:affiliation | Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, 08071 Barcelona, Spain. | lld:pubmed |
| pubmed-article:10768928 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10768928 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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