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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2000-6-13
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pubmed:databankReference |
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pubmed:abstractText |
One representative recombinant clone encoding Klebsiella pneumoniae O5-antigen lipopolysaccharide (LPS) was found upon screening for serum resistance in a cosmid-based genomic library of K. pneumoniae KT769 (O5:K57) introduced into Escherichia coli DH5alpha. A total of eight open reading frames (wb(O5) gene cluster) were necessary to produce K. pneumoniae O5-antigen LPS in E. coli K-12. The enzymatic activities proposed for the wb(O5) gene cluster are in agreement with the activities proposed for the biosynthesis of K. pneumoniae O5-antigen LPS. Using the complete DNA sequence of the K. pneumoniae wb(O5) gene cluster, we obtained (by single or double recombination) genetically well-characterized mutants devoid only of this O5-antigen LPS. Finally, using these O5(-) mutants and the corresponding wild-type strains or complemented mutants with the wb(O5) gene cluster (O5(+) strains), we found that the presence of K. pneumoniae O5-antigen LPS is essential for some pathogenic features like serum resistance, adhesion to uroepithelial cells, and colonization (experimental infections) of the urinary tract in rats.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-10074083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-1587619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-1709919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-1889047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-2569162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-2982796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-3316026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-3519463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-3744547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-3838905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-388439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-4635691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-4778906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-6176137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-6409884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-7108955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-7504166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-7536735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-7542987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-7677991,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-9250780,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10768928-9854064
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2435-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10768928-Amino Acid Sequence,
pubmed-meshheading:10768928-Animals,
pubmed-meshheading:10768928-Base Sequence,
pubmed-meshheading:10768928-Cloning, Molecular,
pubmed-meshheading:10768928-DNA, Bacterial,
pubmed-meshheading:10768928-Escherichia coli,
pubmed-meshheading:10768928-Female,
pubmed-meshheading:10768928-Genes, Bacterial,
pubmed-meshheading:10768928-Klebsiella pneumoniae,
pubmed-meshheading:10768928-Molecular Sequence Data,
pubmed-meshheading:10768928-Multigene Family,
pubmed-meshheading:10768928-Mutagenesis,
pubmed-meshheading:10768928-O Antigens,
pubmed-meshheading:10768928-Open Reading Frames,
pubmed-meshheading:10768928-Rats,
pubmed-meshheading:10768928-Rats, Wistar,
pubmed-meshheading:10768928-Sequence Analysis, DNA
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pubmed:year |
2000
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pubmed:articleTitle |
Cloning and sequencing of the Klebsiella pneumoniae O5 wb gene cluster and its role in pathogenesis.
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pubmed:affiliation |
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, 08071 Barcelona, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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