Source:http://linkedlifedata.com/resource/pubmed/id/10767381
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2000-5-16
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| pubmed:abstractText |
A novel cell line, named LSA, has been obtained, stabilized, and characterized from a human liposarcoma. These cells have morphological and biochemical features strongly resembling the adipocytes and were able to grow in the Ham's F12 medium, in presence or absence of FCS. A conditioned medium (LSA-CM) was obtained by growing the LSA cells in the F12 medium in the absence of FCS. LSA-CM had cytostatic and cytotoxic effects (apoptosis and necrosis) associated with down-regulation of c-myc and upregulation of p53 in several human cell lines (breast, lung, glioblastoma, etc. ). The MCF-7 and glioblastoma cells were killed by LSA-CM in 5-6 days, whereas the same cells were killed by LSA-CM co-incubated with low doses of cisplatin in 30 h. LSA-CM peri-tumoral injections for 15 days in Balb-c-fc3H mice affected by mammary tumors, resulted in the rapid disruption of tumors and absence of metastases. In contrast, in the untreated animals the tumor masses were 4 times larger than initial lesions, and numerous metastases were found in the lungs. The toxicity analysis of LSA-CM, performed on three different animal species, showed that LSA-CM is absolutely free of acute, subacute, and subchronic toxicity. The possible use of LSA-CM/cisplatin for cancer treatment is discussed.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:issn |
1021-335X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
7
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
629-37
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| pubmed:dateRevised |
2005-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10767381-Animals,
pubmed-meshheading:10767381-Apoptosis,
pubmed-meshheading:10767381-Breast Neoplasms,
pubmed-meshheading:10767381-Cell Division,
pubmed-meshheading:10767381-Cisplatin,
pubmed-meshheading:10767381-Culture Media, Conditioned,
pubmed-meshheading:10767381-Female,
pubmed-meshheading:10767381-Glioblastoma,
pubmed-meshheading:10767381-Guinea Pigs,
pubmed-meshheading:10767381-Humans,
pubmed-meshheading:10767381-Liposarcoma,
pubmed-meshheading:10767381-Lung Neoplasms,
pubmed-meshheading:10767381-Mammary Neoplasms, Experimental,
pubmed-meshheading:10767381-Mice,
pubmed-meshheading:10767381-Mice, Inbred C3H,
pubmed-meshheading:10767381-Mutagenicity Tests,
pubmed-meshheading:10767381-Necrosis,
pubmed-meshheading:10767381-Neoplasm Metastasis,
pubmed-meshheading:10767381-Salmonella typhimurium,
pubmed-meshheading:10767381-Skin,
pubmed-meshheading:10767381-Tumor Cells, Cultured,
pubmed-meshheading:10767381-Tumor Suppressor Protein p53
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| pubmed:articleTitle |
A conditioned medium from a human liposarcoma-derived cell line induces p53-dependent apoptosis in several tumor cell lines.
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| pubmed:affiliation |
National Cancer Institute, Fondazione Pascale, 80110 Naples, Italy.
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| pubmed:publicationType |
Journal Article
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