Linked Life Data

10766413

Source:http://linkedlifedata.com/resource/pubmed/id/10766413

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-6-8
pubmed:abstractText
With a view to the possible use of copper(II)-*OH inactivating ligand (OIL) complexes as regulators of inflammation, the reactivity of the copper(II)-ascorbate system with hydrogen peroxide has been investigated in the presence of three key substances: histidine (the main copper(II) low molecular mass ligand in extracellular fluid), salicylic acid (the well-known nonsteroidal antiinflammatory drug, previously shown to be potentiated by copper(II) in animal models of inflammation), and anthranilic acid (an inactive substance by itself, known to be activated by copper(II) in the same models) at physiological pH (7.4) and inflammatory pH (5.5). Such substances may affect the amount of TBARS detected in solution following copper-mediated Fenton-like reactions through three distinct mechanisms: (i) by decreasing the Cu(II)/Cu(I) redox potential, i.e. at the expense of *OH radical production, (ii) by scavenging *OH radicals in the body of the solution, and/or (iii) by acting as a true OIL, i.e. at the expense of *OH detection. Redox potential measurements of initial solutions have been performed in parallel to TBARS determinations to help discriminate between different ligand influences. Computer-aided speciation has been used to understand the role of copper(II) distribution on the ligand effects characterised. Contrary to previous interpretations, histidine has been found to mainly affect *OH production by lowering the redox potential of the Cu(II)/Cu(I) couple. Salicylate, which has no effect on *OH production, has been confirmed to mainly scavenge *OH radicals in the body of the solution. Anthranilate, which both increases *OH production and decreases *OH detection, behaves as a potential OIL. These results tend to confirm our previous hypothesis that copper potentiation of antiinflammatory substances is indirect, i.e. independent of any interaction between metal and drug, whereas copper activation of substances that are inactive by themselves results from specific metal-substance interactions taking place at inflammatory sites.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1071-5762
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
451-61
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Copper--ligand interactions and the physiological free radical processes. Part 3. Influence of histidine, salicylic acid and anthranilic acid on copper-driven Fenton chemistry in vitro.
pubmed:affiliation
Equipe de Chimie Bioinorganique Médicale, ICMPS--CNRS FR1744, Université Paul Sabatier, Toulouse, France.
pubmed:publicationType
Journal Article