Source:http://linkedlifedata.com/resource/pubmed/id/10764681
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2000-4-27
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| pubmed:abstractText |
A novel lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) was recently identified in bovine aortic endothelial cells. It is strongly suggested to have a potential role in the initiation and development of atherosclerosis. In this study, we have isolated cDNA clones encoding the rabbit homologue of LOX-1 by screening a rabbit placenta cDNA library. In amino acid sequence and domain structure organization, the rabbit LOX-1 is highly conserved with the human counterpart. Transfection of rabbit LOX-1 cDNA to HEK-293 cells confers on them the activity to bind and internalize oxidized low density lipoprotein. Rabbit LOX-1 was identified as a 45-kDa protein by Western blot analysis with a specific monoclonal antibody. Notably, analyses by reverse transcription-polymerase chain reaction and Western blot revealed that LOX-1 was accumulated in 8-week-old Watanabe heritable hyperlipidemic rabbit aortas compared with normal rabbit aortas. Immunostaining confirmed that the augmented expression of LOX-1 was primarily localized within the intima at the earliest stages of atherogenesis. The most prominent staining was in the endothelial cells of lesions. Furthermore, the distinctive staining of LOX-1 was identified in the endothelium of non-lesion areas of Watanabe heritable hyperlipidemic rabbit aortas. Taken together, these findings support the possibility that LOX-1 might be involved in the initiation of atherosclerosis.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/OLR1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Oxidized LDL,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class E
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
1079-5642
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
20
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1107-15
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10764681-Amino Acid Sequence,
pubmed-meshheading:10764681-Animals,
pubmed-meshheading:10764681-Aorta,
pubmed-meshheading:10764681-Arteriosclerosis,
pubmed-meshheading:10764681-CHO Cells,
pubmed-meshheading:10764681-Cattle,
pubmed-meshheading:10764681-Cricetinae,
pubmed-meshheading:10764681-DNA, Complementary,
pubmed-meshheading:10764681-Endothelium, Vascular,
pubmed-meshheading:10764681-Female,
pubmed-meshheading:10764681-Gene Expression Regulation,
pubmed-meshheading:10764681-Humans,
pubmed-meshheading:10764681-Hyperlipidemias,
pubmed-meshheading:10764681-Immunohistochemistry,
pubmed-meshheading:10764681-Male,
pubmed-meshheading:10764681-Mice,
pubmed-meshheading:10764681-Molecular Sequence Data,
pubmed-meshheading:10764681-Pregnancy,
pubmed-meshheading:10764681-Rabbits,
pubmed-meshheading:10764681-Rats,
pubmed-meshheading:10764681-Receptors, LDL,
pubmed-meshheading:10764681-Receptors, Oxidized LDL,
pubmed-meshheading:10764681-Scavenger Receptors, Class E,
pubmed-meshheading:10764681-Sequence Alignment,
pubmed-meshheading:10764681-Transfection
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| pubmed:year |
2000
|
| pubmed:articleTitle |
Increased expression of lectin-like oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits.
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| pubmed:affiliation |
National Cardiovascular Center Research Institute, Suita, Osaka, Japan.
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| pubmed:publicationType |
Journal Article,
Comparative Study
|