Source:http://linkedlifedata.com/resource/pubmed/id/10763582
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2000-5-18
|
pubmed:abstractText |
Protein tyrosine kinase (PTK) signaling pathways play important roles in ischemia/reperfusion (I/R) or hypoxia/reoxygenation (H/R) injuries. Inhibition of PTK activation can protect against I/R- or H/R-induced damages. As one part of our work for seeking bioactive compounds from natural sources against I/R or H/R, in the present study we examined the effects of 54 compounds purified from various traditional Chinese herbs on H/R-induced PTK activation by means of an in vitro H/R model in cultured human umbilical vein endothelial cells (HUVEC). The results demonstrated that an increase in PTK activation was induced after 2 h of reoxygenation. Compounds 2 (macrostemososide A), 3 (laxogenin-3-O-beta-D-xylopyranosyl-(1-->4)-alpha-L-arabinopyra nosyl- (1-->6)-beta-D-glucopyranoside), 4 (chinenoside II), 7 (ginsenoside-Rd), 52 (icariin), 53 (icariside), and 54 (icaritin) showed relatively obvious inhibition on this H/R-induced PTK activation. Compounds 5 (beta-sitosterol) and 6 (daucosterine), especially 5, completely blocked such an increased activation of PTK induced by H/R. On the contrary, compound 29 (isocumarine) significantly promoted PTK activation further. Moreover, the effects of these compounds on PTK activation were dose-dependent.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0032-0943
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
66
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
114-8
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:10763582-Cell Hypoxia,
pubmed-meshheading:10763582-Cells, Cultured,
pubmed-meshheading:10763582-Drugs, Chinese Herbal,
pubmed-meshheading:10763582-Endothelium, Vascular,
pubmed-meshheading:10763582-Enzyme Activation,
pubmed-meshheading:10763582-Enzyme Inhibitors,
pubmed-meshheading:10763582-Humans,
pubmed-meshheading:10763582-Perfusion,
pubmed-meshheading:10763582-Protein-Tyrosine Kinases,
pubmed-meshheading:10763582-Reperfusion Injury,
pubmed-meshheading:10763582-Umbilical Veins
|
pubmed:year |
2000
|
pubmed:articleTitle |
Screening of anti-hypoxia/reoxygenation agents by an in vitro model. Part 1: Natural inhibitors for protein tyrosine kinase activated by hypoxia/reoxygenation in cultured human umbilical vein endothelial cells.
|
pubmed:affiliation |
Section of Cellular Physiological Chemistry, Graduate School, Tokyo Medical and Dental University, Japan.
|
pubmed:publicationType |
Journal Article,
In Vitro
|