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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2000-6-21
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pubmed:abstractText |
Mammalian synaptotagmins constitute a multigene family of at least 11 membrane proteins. We have characterized synaptotagmin IV using antibodies directed against the C2A domain of the protein. Antibodies reacted specifically with a protein band that migrated as a 41-44 kDa doublet. Synaptotagmin IV expression was regulated throughout development. A strong decrease in the amount detected by Western blotting occurred between postnatal day 5 and adulthood, in agreement with studies on the expression of synaptotagmin IV transcripts. In subcellular fractionation, synaptotagmin IV was not detected in the synaptic vesicle-enriched fraction. Immunofluorescence microscopy was concordant with this finding. In 6-day-old rat cerebellum and cultured hippocampal neurons the subcellular distribution of synaptotagmin IV was clearly different from that of synaptotagmin I. Synaptotagmin IV displayed a punctate non-polarized distribution on neuronal extensions, whereas synaptotagmin I staining was essentially synaptic. Synaptotagmin IV staining was also observed in the soma in strong perinuclear fluorescent puncta superimposed on that of Golgi/TGN markers. Furthermore, synaptotagmin IV was seen in the proximal part of the growth cone domain and not in the microfilament-rich region which includes filopodia. Co-localizations with the adhesion molecules vinculin and zyxin at the proximal part of growth cones were observed. Synaptotagmin IV may thus be involved in the regulation of specific membrane-trafficking pathways during brain development.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptotagmin I,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptotagmins,
http://linkedlifedata.com/resource/pubmed/chemical/Syt1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Syt4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Vinculin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0953-816X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1294-302
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10762358-Animals,
pubmed-meshheading:10762358-Antibodies,
pubmed-meshheading:10762358-Blotting, Western,
pubmed-meshheading:10762358-CHO Cells,
pubmed-meshheading:10762358-Calcium-Binding Proteins,
pubmed-meshheading:10762358-Cell Compartmentation,
pubmed-meshheading:10762358-Cricetinae,
pubmed-meshheading:10762358-Cytoplasm,
pubmed-meshheading:10762358-Genes, Reporter,
pubmed-meshheading:10762358-Golgi Apparatus,
pubmed-meshheading:10762358-Green Fluorescent Proteins,
pubmed-meshheading:10762358-Growth Cones,
pubmed-meshheading:10762358-Hippocampus,
pubmed-meshheading:10762358-Indicators and Reagents,
pubmed-meshheading:10762358-Intracellular Membranes,
pubmed-meshheading:10762358-Luminescent Proteins,
pubmed-meshheading:10762358-Membrane Glycoproteins,
pubmed-meshheading:10762358-Metalloproteins,
pubmed-meshheading:10762358-Nerve Tissue Proteins,
pubmed-meshheading:10762358-Neurons,
pubmed-meshheading:10762358-Rabbits,
pubmed-meshheading:10762358-Rats,
pubmed-meshheading:10762358-Subcellular Fractions,
pubmed-meshheading:10762358-Synaptic Vesicles,
pubmed-meshheading:10762358-Synaptotagmin I,
pubmed-meshheading:10762358-Synaptotagmins,
pubmed-meshheading:10762358-Transfection,
pubmed-meshheading:10762358-Vinculin
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pubmed:year |
2000
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pubmed:articleTitle |
Synaptotagmin I and IV define distinct populations of neuronal transport vesicles.
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pubmed:affiliation |
INSERM U 464, Institut Fédératif Jean Roche, Université de la Méditerranée, Faculté de Médecine, Marseille Cedex 20, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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