Linked Life Data

10760302

Source:http://linkedlifedata.com/resource/pubmed/id/10760302

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2000-5-17
pubmed:databankReference
pubmed:abstractText
Members of the nuclear receptor superfamily are thought to activate transcription by recruitment of one or more recently identified coactivator complexes. Here we demonstrate that both peroxisome proliferator-activated receptor binding protein (PBP) and steroid receptor coactivator-1 (SRC-1) are required for ligand-dependent transcription of transiently transfected and chromosomally integrated reporter genes by the estrogen receptor (ER) and retinoic acid receptor (RAR). To examine ligand-dependent interactions between nuclear receptors and specific coactivators in living cells, these proteins were tagged with cyan (CFP) and yellow (YFP) mutants of the green fluorescent protein. Fluorescence resonance energy transfer (FRET) from the CFP to the YFP indicated interaction between the receptor and coactivator. CFP fusions to RAR or its ligand-binding domain exhibited rapid ligand-dependent FRET to YFP-tagged nuclear receptor interaction domains of the coactivators SRC-1 and PBP. The ER-ligand-binding domain, unlike RAR, also exhibited some basal interaction with coactivators in unstimulated cells that was abolished by the receptor antagonists tamoxifen or ICI182,780. Inhibition of FRET by tamoxifen but not ICI182,780 could be reversed by estradiol, whereas estradiol-enhanced FRET could not be inhibited by either antagonist, indicating that ligand effects can show varying degrees of hysteresis. These findings suggest that ligand-dependent transcriptional activities of the RAR and ER require concurrent or sequential recruitment of SRC-1 and PBP-containing coactivator complexes.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-10235266, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-10319811, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-10368774, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-10490106, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-12876789, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-7481822, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-8521507, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-8616895, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-8710870, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9192892, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9192902, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9278050, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9338790, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9422508, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9430642, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9445474, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9445475, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9482670, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9618493, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9637681, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9640540, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9653119, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9731708, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9744270, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9744281, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9808622, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9808623, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9875847, http://linkedlifedata.com/resource/pubmed/commentcorrection/10760302-9950689
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Histone Acetyltransferases, http://linkedlifedata.com/resource/pubmed/chemical/Ligands, http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins, http://linkedlifedata.com/resource/pubmed/chemical/MED1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Mediator Complex Subunit 1, http://linkedlifedata.com/resource/pubmed/chemical/NCOA1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Coactivator 1, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4363-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
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