Source:http://linkedlifedata.com/resource/pubmed/id/10759189
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2000-4-26
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| pubmed:abstractText |
The adenomatous polyposis coli (APC) gene, a member of the Wingless/Wnt signal transduction pathway, has been implicated in the development of medulloblastomas in Turcot's syndrome. beta-catenin also functions in this highly conserved signaling pathway and is instrumental in growth and development. Mutations in either APC or beta-catenin can stabilize beta-catenin protein. Stabilized beta-catenin complexes with Tcf/Lef transcription factors and moves from the cytoplasm into the nucleus where it regulates the transcription of c-Myc and other genes. Nuclear localization of beta-catenin therefore implies activation of the signaling pathway. We have analyzed the subcellular localization of beta-catenin in 51 sporadic medulloblastomas and in 1 medulloblastoma arising in a patient with Turcot's syndrome. Nuclear beta-catenin staining was present in 9 of the sporadic tumors (18%) and in the 1 medulloblastoma from a Turcot's patient. The remaining 41 cases did not show nuclear staining. This confirms earlier observations that Wingless/Wnt signaling is involved in a subset of sporadic medulloblastomas. We also examined 48 glial and meningeal CNS tumors, all of which were negative for nuclear beta-catenin. Exon 3 of beta-catenin was sequenced in 6 of the 9 sporadic medulloblastomas with nuclear beta-catenin staining. Five of the 6 tumors sequenced had mutations affecting highly conserved beta-catenin phosphorylation sites involved in protein stability. These data suggest a simple immunohistochemical method to screen for beta-catenin mutations in medulloblastomas.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CTNNB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0022-3069
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
59
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
333-7
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10759189-Aged,
pubmed-meshheading:10759189-Binding Sites,
pubmed-meshheading:10759189-Cell Nucleus,
pubmed-meshheading:10759189-Central Nervous System Neoplasms,
pubmed-meshheading:10759189-Cytoplasm,
pubmed-meshheading:10759189-Cytoskeletal Proteins,
pubmed-meshheading:10759189-DNA Mutational Analysis,
pubmed-meshheading:10759189-Exons,
pubmed-meshheading:10759189-Fetal Diseases,
pubmed-meshheading:10759189-Humans,
pubmed-meshheading:10759189-Immunohistochemistry,
pubmed-meshheading:10759189-Medulloblastoma,
pubmed-meshheading:10759189-Middle Aged,
pubmed-meshheading:10759189-Mutation,
pubmed-meshheading:10759189-Phosphorylation,
pubmed-meshheading:10759189-Polymerase Chain Reaction,
pubmed-meshheading:10759189-Proto-Oncogene Proteins,
pubmed-meshheading:10759189-Signal Transduction,
pubmed-meshheading:10759189-Survival Rate,
pubmed-meshheading:10759189-Trans-Activators,
pubmed-meshheading:10759189-Wnt Proteins,
pubmed-meshheading:10759189-Zebrafish Proteins,
pubmed-meshheading:10759189-beta Catenin
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| pubmed:year |
2000
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| pubmed:articleTitle |
Nuclear localization and mutation of beta-catenin in medulloblastomas.
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| pubmed:affiliation |
Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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