Source:http://linkedlifedata.com/resource/pubmed/id/10757773
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2000-7-13
|
| pubmed:abstractText |
In plants with a gametophytic self-incompatibility system the specificity of the pollen is determined by the haploid genotype at the self-incompatibility (S) locus. In certain crosses this can lead to the exclusion of half the gametes from the male parent carrying a particular S-allele. This leads to pronounced segregation distortion for any genetic markers that are linked to the S-locus. We have used this approach to identify T-DNA insertions carrying a maize transposable element that are linked to the S-locus of Petunia hybrida. A total of 83 T-DNA insertions were tested for segregation distortion of the selectable marker used during transformation with Agrobacterium. Segregation distortion was observed for 12 T-DNA insertions and at least 8 of these were shown to be in the same linkage group by intercrossing. This indicates that differential transmission of a single locus (S) is probably responsible for all of these examples of T-DNA segregation distortion. The identification of selectable markers in coupling with a functional S-allele will allow the preselection of recombination events around the S-locus in petunia. Our approach provides a general method for identifying transgenes that are linked to gametophytic self-incompatibility loci and provides an opportunity for transposon tagging of the petunia S-locus.
|
| pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-16576988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-16593587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-1766433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-2101314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-2152130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-6388563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-7509041,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-7756690,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-7902614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-8608599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-8612266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-8676880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-8843939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10757773-9061954
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0016-6731
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
154
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1323-33
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading | |
| pubmed:year |
2000
|
| pubmed:articleTitle |
Segregation distortion of T-DNA markers linked to the self-incompatibility (S) locus in Petunia hybrida.
|
| pubmed:affiliation |
Sainsbury Laboratory, John Innes Centre, Norwich NR4 7UH, United Kingdom.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|