Linked Life Data

10756306

Source:http://linkedlifedata.com/resource/pubmed/id/10756306

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-8-23
pubmed:abstractText
In order to evaluate whether or not polyethylene terephthalate coated with pyrolytic carbon and collagen (PET+PC) favors inflammatory or hyperplastic reactions, the expression of mRNAs specific for interleukin-6 (IL-6), platelet-derived growth factor-A (PDGF-A), PDGF-B, transforming growth factor-beta1 (TGF-beta1), and TGF-beta2 were tested in vitro by cultured human umbilical vein endothelial cells (HUVEC). The cultures were put in contact with PET+PC for 1, 24, 48, and 72 h. The same cells cultured on polystyrene without biomaterials were tested as negative controls; cultures incubated with LPS were the positive control. The expression of mRNAs was evaluated by RT-PCR with specific primers. PET+PC did not determine any differences in the expression of IL-6-specific mRNA at any of the incubation times compared to the negative control while LPS (the positive control) induced expression after 24, 48, and 72 h. PET+PC induced a more precocious expression of mRNA specific for PDGF-A than did the negative control; however, the expression no longer was present after 48 h while in the negative control the expression stopped after 72 h. PET+PC induced a less frequent expression of PDGF-B-specific mRNA than did the negative control and LPS, especially after 24 h. PET+PC induced a later expression of TGF-beta2-specific mRNA than did the negative control and a less frequent expression of mRNA specific for TGF-beta1 after 24 and 72 h.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9304
pubmed:author
pubmed:copyrightInfo
Copyright 2000 John Wiley & Sons, Inc.
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
50
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
483-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10756306-Biocompatible Materials, pubmed-meshheading:10756306-Carbon, pubmed-meshheading:10756306-Cell Adhesion, pubmed-meshheading:10756306-Cells, Cultured, pubmed-meshheading:10756306-Collagen, pubmed-meshheading:10756306-Cytokines, pubmed-meshheading:10756306-Endothelium, Vascular, pubmed-meshheading:10756306-Gene Expression Regulation, pubmed-meshheading:10756306-Humans, pubmed-meshheading:10756306-Interleukin-6, pubmed-meshheading:10756306-Platelet-Derived Growth Factor, pubmed-meshheading:10756306-Polyethylene Terephthalates, pubmed-meshheading:10756306-RNA, Messenger, pubmed-meshheading:10756306-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10756306-Transcription, Genetic, pubmed-meshheading:10756306-Transforming Growth Factor beta, pubmed-meshheading:10756306-Umbilical Veins
pubmed:year
2000
pubmed:articleTitle
Cytokine expression in vitro by cultured human endothelial cells in contact with polyethylene terephthalate coated with pyrolytic carbon and collagen.
pubmed:affiliation
Istituti Ortopedici Rizzoli, via di Barbiano 1/10, 40136 Bologna, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't