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rdf:type |
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lifeskim:mentions |
umls-concept:C0012751,
umls-concept:C0012854,
umls-concept:C0013126,
umls-concept:C0040649,
umls-concept:C0205112,
umls-concept:C0332158,
umls-concept:C0337076,
umls-concept:C0441472,
umls-concept:C0920533,
umls-concept:C1417098,
umls-concept:C1533691
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pubmed:issue |
9
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pubmed:dateCreated |
2000-6-20
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pubmed:abstractText |
The pathways for selective transcriptional repression of methylated DNA templates by the methyl-CpG-binding protein MeCP2 have been investigated using a purified in vitro transcription system that does not assemble chromatin. MeCP2 selectively inhibits transcription complex assembly on methylated DNA but does not destabilize a pre-assembled transcription complex. MeCP2 functions to repress transcription at a distance of >500 bp from the transcription start site. The transcription repression domain (TRD) of MeCP2 will repress transcription in vitro when fused to a heterologous Gal4 DNA-binding domain. The TRD associates with TFIIB. Exogenous TFIIB does not relieve transcriptional repression established by either intact MeCP2 or a Gal4-TRD fusion protein under these in vitro conditions, nor does the addition of histone deacetylase inhibitors. We find that the transcriptional repression established by both MeCP2 and the Gal4-TRD fusion protein in vitro also correlates with selective assembly of large nucleoprotein complexes. The formation of such complexes reflects a local concentration of DNA-bound transcriptional repressor that may stabilize a state of repression even in the presence of exogenous transcriptional machinery.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10319871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10353812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10471499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10471500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10508514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10518942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10521337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10577905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10581239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-10589672,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-1408825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-1613793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-1717265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-1741245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-2405489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-2545524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-3029768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-7565754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-7732579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-7876175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-8177735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-8406006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-8524323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-8563762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9038338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9285713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9385841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9395433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9601009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9601010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9611234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9620779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9620804,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9632760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9710634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9724627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9774669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9916800,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10756192-9988266
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1921-8
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:10756192-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:10756192-DNA,
pubmed-meshheading:10756192-DNA Methylation,
pubmed-meshheading:10756192-DNA-Binding Proteins,
pubmed-meshheading:10756192-Glutathione Transferase,
pubmed-meshheading:10756192-Methyl-CpG-Binding Protein 2,
pubmed-meshheading:10756192-Recombinant Fusion Proteins,
pubmed-meshheading:10756192-Repressor Proteins,
pubmed-meshheading:10756192-Transcription, Genetic,
pubmed-meshheading:10756192-Transcription Factor TFIIB,
pubmed-meshheading:10756192-Transcription Factors
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pubmed:year |
2000
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pubmed:articleTitle |
MeCP2 driven transcriptional repression in vitro: selectivity for methylated DNA, action at a distance and contacts with the basal transcription machinery.
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pubmed:affiliation |
Laboratory of Molecular Embryology, National Institute of Child Heath and Human Development, NIH, Building 18T, Room 106, Bethesda, MD 20892-5431, USA.
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pubmed:publicationType |
Journal Article
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