Linked Life Data

10754270

Source:http://linkedlifedata.com/resource/pubmed/id/10754270

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-5-25
pubmed:abstractText
Previous investigations of our laboratory have shown that 7-ketocholesterol was a potent inducer of apoptosis involving a release of cytochrome c into the cytosol, and a lipid peroxidation process that could be the consequence of a production of radical oxygen species. According to these considerations, we asked whether some antioxidants were able to counteract 7-ketocholesterol-induced apoptosis, and whether prevention of cell death was associated with the impairment of mitochondrial events implied in the commitment to apoptosis, i.e., opening of the mitochondrial megachannels leading to the loss of the mitochondrial transmembrane potential (DeltaPsim), and release of cytochrome c from mitochondria into the cytosol. To this end, we studied the effects of glutathione (15 mM), N-acetylcysteine (15 mM), vitamin E (100 microM), vitamin C (50 microM) and melatonin (1 mM) on U937 cells treated with 7-ketocholesterol (40 microg/ml). Only glutathione, N-acetylcysteine, and vitamin E prevented apoptosis measured by the occurrence of cells with condensed and/or fragmented nuclei, as well as the loss of DeltaPsim, and the release of cytochrome c. However, all the antioxidants used were potent inhibitors of the production of O(2)(*) occuring under treatment with 7-ketocholesterol. Collectively, our data demonstrate that impairment of apoptosis by glutathione, N-acetylcysteine, and vitamin E correlates with the prevention of mitochondrial dysfunctions, and they underline that the ability of antioxidants to counteract 7-ketocholesterol-induced apoptosis does not only depend on their capability to inhibit the production of O(2)(*).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0891-5849
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
28
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
743-53
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10754270-Acetylcysteine, pubmed-meshheading:10754270-Antioxidants, pubmed-meshheading:10754270-Apoptosis, pubmed-meshheading:10754270-Ascorbic Acid, pubmed-meshheading:10754270-Cytochrome c Group, pubmed-meshheading:10754270-Cytosol, pubmed-meshheading:10754270-Free Radicals, pubmed-meshheading:10754270-Glutathione, pubmed-meshheading:10754270-Humans, pubmed-meshheading:10754270-Intracellular Membranes, pubmed-meshheading:10754270-Ketocholesterols, pubmed-meshheading:10754270-Kinetics, pubmed-meshheading:10754270-Melatonin, pubmed-meshheading:10754270-Membrane Potentials, pubmed-meshheading:10754270-Microscopy, Electron, pubmed-meshheading:10754270-Mitochondria, pubmed-meshheading:10754270-Superoxides, pubmed-meshheading:10754270-U937 Cells, pubmed-meshheading:10754270-Vitamin E
pubmed:year
2000
pubmed:articleTitle
Impairment with various antioxidants of the loss of mitochondrial transmembrane potential and of the cytosolic release of cytochrome c occuring during 7-ketocholesterol-induced apoptosis.
pubmed:affiliation
Inserm-U498 (Métabolisme des lipoprotéines humaines et interactions vasculaires), CHU/Hôpital du Bocage, BP 1542, 21034, Dijon Cedex, France. Gerard.Lizard@u-bourgogne.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't