Linked Life Data

10753848

Source:http://linkedlifedata.com/resource/pubmed/id/10753848

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2000-5-5
pubmed:abstractText
We studied lineage-specific chimerism and minimal residual disease (MRD) in sequential posttransplant samples from 55 patients who underwent unmanipulated (n = 44) or partially T-cell-depleted (n = 11) allogeneic bone marrow transplantation (BMT) for chronic myeloid leukemia (CML). Chimerism was assessed by polymerase chain reaction (VNTR [variable number of tandem repeats]-PCR) analysis in highly purified CD19+, CD3+, CD15+, and CD56+ cell fractions, whereas MRD was investigated in whole blood by reverse transcriptase-PCR (RT-PCR) of both p210(BCR-ABL) and p190(BCR-ABL) hybrid transcripts. Of 55 patients, 14 (including 6 T-cell-depleted patients) had cytogenetic relapse at 5-80 months and progressed to hematologic relapse, while 41 patients remained in prolonged cytogenetic remission 12-107 months post-BMT. Before leukemia recurrence, patients in the relapse group showed a consistent evolution pattern sequentially featured by persistent p210(BCR-ABL) positivity, increasing mixed chimerism (MC) in myeloid cells, p190(BCR-ABL) positivity, and, finally, cytogenetic relapse. Myeloid MC preceded cytogenetic relapse by 2-12 months, whereas p190(BCR/ABL) was detected 1-6 months prior to cytogenetic relapse in 11 patients and concomitant with cytogenetic relapse in 3 patients. In the remission group, all patients invariably tested negative for p190(BCR-ABL); 10 patients tested positive for p210(BCR-ABL) at variable time-points but showed persistent full donor chimerism (DC), whereas 31 patients tested p210(BCR-ABL) negative and displayed full DC or transient MC due to the persistence of recipient T cells. Two patients in the relapse group were successfully reinduced into molecular remission with donor lymphocyte infusion. Sequential molecular analysis after such treatment showed the inverse pattern to that observed prior to relapse, ie, progressive disappearance of p190(BCR-ABL) transcripts, conversion of myeloid chimerism to donor type, and, finally, p210(BCR-ABL) negativity. We conclude that lineage-specific chimerism and p190(BCR-ABL) messenger RNA (mRNA) analyses contribute a better characterization of CML evolution after BMT and enable early identification of patients at the highest risk of relapse. (Blood. 2000;95:2659-2665)
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2659-65
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:10753848-Adolescent, pubmed-meshheading:10753848-Adult, pubmed-meshheading:10753848-Bone Marrow Transplantation, pubmed-meshheading:10753848-Cell Lineage, pubmed-meshheading:10753848-Child, pubmed-meshheading:10753848-Female, pubmed-meshheading:10753848-Fusion Proteins, bcr-abl, pubmed-meshheading:10753848-Humans, pubmed-meshheading:10753848-Leukemia, Myelogenous, Chronic, BCR-ABL Positive, pubmed-meshheading:10753848-Male, pubmed-meshheading:10753848-Middle Aged, pubmed-meshheading:10753848-Neoplasm, Residual, pubmed-meshheading:10753848-Predictive Value of Tests, pubmed-meshheading:10753848-Recurrence, pubmed-meshheading:10753848-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10753848-Transplantation, Homologous, pubmed-meshheading:10753848-Transplantation Chimera
pubmed:year
2000
pubmed:articleTitle
Molecular analysis of lineage-specific chimerism and minimal residual disease by RT-PCR of p210(BCR-ABL) and p190(BCR-ABL) after allogeneic bone marrow transplantation for chronic myeloid leukemia: increasing mixed myeloid chimerism and p190(BCR-ABL) detection precede cytogenetic relapse.
pubmed:affiliation
Hematology Department of Reina Sofía Hospital, Córdoba, Spain. josefina.serrano@iname.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't