Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-8-16
pubmed:abstractText
Multilocus sequence typing (MLST) is a highly discriminatory molecular typing method that defines isolates of bacterial pathogens using the sequences of approximately 450-bp internal fragments of seven housekeeping genes. This technique has been applied to 575 isolates of Streptococcus pneumoniae and identifies a number of discrete clonal complexes. These clonal complexes are typically represented by a single group of isolates sharing identical alleles at all seven loci, plus single-locus variants that differ from this group at only one out of the seven loci. As MLST is highly discriminatory, the members of each clonal complex can be assumed to have a recent common ancestor, and the molecular events that give rise to the single-locus variants can be used to estimate the relative contributions of recombination and mutation to clonal divergence. By comparing the sequences of the variant alleles within each clonal complex with the allele typically found within that clonal complex, we estimate that recombination has generated new alleles at a frequency approximately 10-fold higher than mutation, and that a single nucleotide site is approximately 50 times more likely to change through recombination than mutation. We also demonstrate how to estimate the average length of recombinational replacements from MLST data.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10365396, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10368953, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10383857, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10463168, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10488179, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10511575, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10555280, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10603483, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-10605111, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-1344989, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-1406254, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-1766389, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-1938920, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-7479835, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-7973728, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-8153626, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-8277244, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-8500868, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-8506277, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-8995060, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-9157250, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-9466257, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-9501229, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-9770535, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-9817864, http://linkedlifedata.com/resource/pubmed/commentcorrection/10747043-9846740
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1439-50
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Estimating recombinational parameters in Streptococcus pneumoniae from multilocus sequence typing data.
pubmed:affiliation
Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, United Kingdom. ed.feil@ceid.ox.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't