|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
2000-5-15
|
|
pubmed:abstractText |
A previously unrecognized mismatch repair activity is described. Extracts of immortalized MSH2-deficient mouse fibroblasts did not correct most single base mispairs. The same extracts carried out efficient repair of A/C mismatches. A/G mispairs were less efficiently corrected and there was no significant repair of A/A. MLH1-defective mouse extracts also repaired an A/C mispair. A/C correction by Msh2(-/-) mouse cell extracts was not affected by antibodies against the PMS2 protein, which inhibited long-patch mismatch repair. A/C repair activity is thus independent of MutSalpha, MutSbeta and MutLalpha. A/C mismatches were corrected 5-fold more efficiently by extracts of Msh2 knockout mouse cells than by comparable extracts prepared from hMSH2- or hMLH1-deficient human cells. MSH2-independent A/C correction by mouse cell extracts did not require a nick in the circular duplex DNA substrate. Repair involved replacement of the A and was associated with the resynthesis of a limited stretch of </=25 bases of DNA.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-1495996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-2734295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-7628019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-7628020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-7823963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-8264607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-8533151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-8636155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-8673133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-8674118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-8811176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-9115419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-9335609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-9353325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-942051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-9443401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747038-9583678
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MLH1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MSH2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Mlh1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Msh2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/MutS Homolog 2 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0261-4189
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
3
|
|
pubmed:volume |
19
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1711-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10747038-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10747038-Animals,
pubmed-meshheading:10747038-Base Pair Mismatch,
pubmed-meshheading:10747038-Base Sequence,
pubmed-meshheading:10747038-Carrier Proteins,
pubmed-meshheading:10747038-Cell Line,
pubmed-meshheading:10747038-DNA Primers,
pubmed-meshheading:10747038-DNA Repair,
pubmed-meshheading:10747038-DNA-Binding Proteins,
pubmed-meshheading:10747038-HeLa Cells,
pubmed-meshheading:10747038-Humans,
pubmed-meshheading:10747038-Mice,
pubmed-meshheading:10747038-Mice, Knockout,
pubmed-meshheading:10747038-MutS Homolog 2 Protein,
pubmed-meshheading:10747038-Neoplasm Proteins,
pubmed-meshheading:10747038-Nuclear Proteins,
pubmed-meshheading:10747038-Proto-Oncogene Proteins
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Efficient repair of A/C mismatches in mouse cells deficient in long-patch mismatch repair.
|
|
pubmed:affiliation |
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts EN6 3LD, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
