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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-5-15
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pubmed:abstractText |
The phosphotyrosine-binding (PTB) domain of the cell fate determinant Numb is involved in the formation of multiple protein complexes in vivo and can bind a diverse array of peptide sequences in vitro. To investigate the structural basis for the promiscuous nature of this protein module, we have determined its solution structure by NMR in a complex with a peptide containing an NMSF sequence derived from the Numb-associated kinase (Nak). The Nak peptide was found to adopt a significantly different structure from that of a GPpY sequence-containing peptide previously determined. In contrast to the helical turn adopted by the GPpY peptide, the Nak peptide forms a beta-turn at the NMSF site followed by another turn near the C-terminus. The Numb PTB domain appears to recognize peptides that differ in both primary and secondary structures by engaging various amounts of the binding surface of the protein. Our results suggest a mechanism through which a single PTB domain might interact with multiple distinct target proteins to control a complex biological process such as asymmetric cell division.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10747019-10338211,
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1505-15
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10747019-Amino Acid Sequence,
pubmed-meshheading:10747019-Animals,
pubmed-meshheading:10747019-Binding Sites,
pubmed-meshheading:10747019-Drosophila Proteins,
pubmed-meshheading:10747019-Juvenile Hormones,
pubmed-meshheading:10747019-Macromolecular Substances,
pubmed-meshheading:10747019-Models, Molecular,
pubmed-meshheading:10747019-Molecular Sequence Data,
pubmed-meshheading:10747019-Mutagenesis, Site-Directed,
pubmed-meshheading:10747019-Peptides,
pubmed-meshheading:10747019-Phosphotyrosine,
pubmed-meshheading:10747019-Protein Binding,
pubmed-meshheading:10747019-Protein Conformation,
pubmed-meshheading:10747019-Protein Structure, Tertiary,
pubmed-meshheading:10747019-Protein-Serine-Threonine Kinases
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pubmed:year |
2000
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