Linked Life Data

10746650

Source:http://linkedlifedata.com/resource/pubmed/id/10746650

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-4-11
pubmed:abstractText
We have recently demonstrated that 17beta-estradiol (E2) opposes cytokine-dependent increase of inducible nitric oxide synthase (iNOS) activity in rat smooth muscle cells and proposed that this effect might be associated to an antiinflammatory activity of this hormone. In the present study, we examine the E2 effects on a well-known in vivo model of inflammation. We show that, in carrageenan treatment of ovariectomized rats, prior exposure to E2 significantly attenuated inflammatory response as measured by histological examination and exudate production. The effect was visible with a single injection of a physiological dose of E2 1 h before the carrageenan treatment and was blocked by coadministration of the estrogen receptor antagonists ICI 182,780 or tamoxifen. This latter observation suggests that the effect is receptor mediated. The mechanisms by which estradiol has beneficial effects in this model of inflammation are unclear: we show that in hormonally treated rats there is a decrease in polymorphonuclear cells migration as shown by cell counting and myeloperoxidase measurement. In addition, E2 pretreatment opposes carrageneen-induced high lipid peroxidation maintaining malondialdehyde activity at control levels. E2 treatment decreases NO production and the activity of iNOS with consequent diminished nitrite synthesis and nitrosine accumulation. Finally, immunohistochemical analysis for poly (ADP-ribose) synthase revealed a positive staining in lungs from carrageenan-treated rats that was blocked by estradiol treatment. We conclude that E2 attenuates the degree of inflammation and tissue damage associated with carrageenan-induced pleurisy in the rat.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1455-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10746650-Animals, pubmed-meshheading:10746650-Anti-Inflammatory Agents, pubmed-meshheading:10746650-Carrageenan, pubmed-meshheading:10746650-Cell Movement, pubmed-meshheading:10746650-Estradiol, pubmed-meshheading:10746650-Female, pubmed-meshheading:10746650-Lipid Peroxides, pubmed-meshheading:10746650-Lung, pubmed-meshheading:10746650-Malondialdehyde, pubmed-meshheading:10746650-Neutrophils, pubmed-meshheading:10746650-Nitric Oxide, pubmed-meshheading:10746650-Nitric Oxide Synthase, pubmed-meshheading:10746650-Nitric Oxide Synthase Type II, pubmed-meshheading:10746650-Ovariectomy, pubmed-meshheading:10746650-Peroxidase, pubmed-meshheading:10746650-Pleurisy, pubmed-meshheading:10746650-Poly(ADP-ribose) Polymerases, pubmed-meshheading:10746650-Rats, pubmed-meshheading:10746650-Rats, Sprague-Dawley
pubmed:year
2000
pubmed:articleTitle
17beta-estradiol antiinflammatory activity in carrageenan-induced pleurisy.
pubmed:affiliation
Institute of Pharmacology, University of Messina, Italy. salvator@www.unime.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't