Linked Life Data

10744707

Source:http://linkedlifedata.com/resource/pubmed/id/10744707

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
14
pubmed:dateCreated
2000-5-8
pubmed:abstractText
Trypanosoma cruzi has a complex mucin gene family of 500 members with hypervariable regions expressed preferentially in vertebrate associated stages of the parasite. In this work, a novel mucin-type gene family is reported, composed of two groups of genes organized in independent tandems and having very short open reading frames. The structures of deduced proteins share the N and C termini but differ in central regions. One group has repeats with the consensus Lys-Asn-Thr(7)-Ser-Thr(3)-Ser(Ser/Lys)-Ala-Pro and the other a Thr-rich sequence of the type Asp-Gln-Thr(17-20)-Asn-Ala-Pro-Ala-Lys-Asp-Thr(5-7)-Asn-Ala-Pro-Ala-L ys. In both cases, expected mature core proteins are around 7 kDa. Both groups, named L and S, respectively, differ in the structure of genomic loci and mRNA, with differential blocks in the 3'-untranslated region. The highest mRNA level for S and L groups are in the epimastigote stage but they show distinct developmentally regulated patterns. Transcripts are short lived and their steady-state abundance is regulated post-transcriptionally with increased mRNA stability in insect stage epimastigote. AU-rich sequences, similar to ARE motives known to cause mRNA instability in higher eukaryotes, are present in the 3'-untranslated region of the transcripts. In transfection experiments this sequence is shown to be functional for the L group destabilizing its mRNA in a stage-specific manner. Furthermore, an effect of this AU-rich region on translation efficiency is shown. To our knowledge, this is the first time that a functional ARE sequence-dependent post-transcriptional regulation mechanism is reported in a lower eukaryote.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10218-27
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10744707-3' Untranslated Regions, pubmed-meshheading:10744707-Amino Acid Sequence, pubmed-meshheading:10744707-Animals, pubmed-meshheading:10744707-Base Composition, pubmed-meshheading:10744707-Base Pairing, pubmed-meshheading:10744707-Base Sequence, pubmed-meshheading:10744707-Cloning, Molecular, pubmed-meshheading:10744707-Conserved Sequence, pubmed-meshheading:10744707-Genes, Protozoan, pubmed-meshheading:10744707-Glycosylation, pubmed-meshheading:10744707-Molecular Sequence Data, pubmed-meshheading:10744707-Mucins, pubmed-meshheading:10744707-Multigene Family, pubmed-meshheading:10744707-Protein Biosynthesis, pubmed-meshheading:10744707-Protein Sorting Signals, pubmed-meshheading:10744707-RNA, Messenger, pubmed-meshheading:10744707-RNA, Protozoan, pubmed-meshheading:10744707-RNA Processing, Post-Transcriptional, pubmed-meshheading:10744707-Sequence Alignment, pubmed-meshheading:10744707-Sequence Homology, Amino Acid, pubmed-meshheading:10744707-Transcription, Genetic, pubmed-meshheading:10744707-Trypanosoma cruzi
pubmed:year
2000
pubmed:articleTitle
AU-rich elements in the 3'-untranslated region of a new mucin-type gene family of Trypanosoma cruzi confers mRNA instability and modulates translation efficiency.
pubmed:affiliation
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, C.C. 30, 1650 San Martín, Pcia. de Buenos Aires, Argentina.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't