Linked Life Data

10743742

Source:http://linkedlifedata.com/resource/pubmed/id/10743742

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-5-12
pubmed:abstractText
The article describes a polymerase chain reaction (PCR) based method for semiquantitative assessment of mRNA expression for chemotactic cytokines in the lower respiratory tract cells. Cytokine mRNA was detected using PCR preceded by reverse transcription of mRNA into cDNA. The procedures of RNA extraction and of reverse transcription-PCR assay are described in the first part of the article. The second part demonstrates optimalisation experiments performed to ensure specificity, accuracy and reproducibility of the RT-PCR assay and discuss the approach to mRNA quantification using normalisation of cytokine mRNA expression to the expression of beta-actin mRNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-2514
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
127-34
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Analysis of chemokine gene expression in lung cells by polymerase chain reaction.
pubmed:affiliation
Department of Clinical Immunology, Medical Faculty of Palacký University, Olomouc, Czech Republic.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't