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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-5-25
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pubmed:abstractText |
1. The muscarinic acetylcholine receptors mediating the contractile response elicited to endogenous acetylcholine released by the selective P2X receptor agonist alpha,beta-methylene ATP (mATP) were investigated in guinea-pig ileum. 2. mATP (0.1 - 30 microM) elicited a concentration-dependent neurogenic contractile response inhibited by tetrodotoxin (TTX) and antagonized by the non-selective muscarinic receptor antagonist N-methylscopolamine (NMS). 3. The contractile response to mATP was pertussis toxin-insensitive, irreversibly antagonized by N-(2-chloroethyl)-4-piperidinyl diphenylacetate (4-DAMP mustard), and unaffected by the muscarinic M(2)/M(4) receptor selective antagonist AF-DX 116 (1 microM). 4. When measured in the presence of histamine and isoproterenol after treatment with 4-DAMP mustard, mATP elicited a pertussis toxin-sensitive contractile response potently antagonized by AF-DX 116. 5. Collectively, our data suggest that endogenous acetylcholine released by mATP can elicit a direct contractile response through the muscarinic M(3) receptor and an indirect contractile response through the muscarinic M(2) receptor by antagonizing the relaxant effects of isoproterenol on histamine induced contraction.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-10082268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-10215678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-10550991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-1328607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-1569923,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-1661840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-1672880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-2172776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-2841607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-3012078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-31960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-3207984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-3978322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-6270235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-6277650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-6489444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-6541517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-7813548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-7965766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-8393516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-8759038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-8930839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-8981565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-8996196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-9283681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-9365220,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-9435187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742302-9755289
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Diphenylacetic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Muscarinic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/N-(2-chloroethyl)-4-piperidinyl...,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methylscopolamine,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidines,
http://linkedlifedata.com/resource/pubmed/chemical/Pirenzepine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Muscarinic M2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Muscarinic,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella,
http://linkedlifedata.com/resource/pubmed/chemical/alpha,beta-methyleneadenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/otenzepad
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
129
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1458-64
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10742302-Adenosine Triphosphate,
pubmed-meshheading:10742302-Animals,
pubmed-meshheading:10742302-Diphenylacetic Acids,
pubmed-meshheading:10742302-Dose-Response Relationship, Drug,
pubmed-meshheading:10742302-Guinea Pigs,
pubmed-meshheading:10742302-Ileum,
pubmed-meshheading:10742302-Male,
pubmed-meshheading:10742302-Muscarinic Antagonists,
pubmed-meshheading:10742302-Muscle Contraction,
pubmed-meshheading:10742302-N-Methylscopolamine,
pubmed-meshheading:10742302-Pertussis Toxin,
pubmed-meshheading:10742302-Piperidines,
pubmed-meshheading:10742302-Pirenzepine,
pubmed-meshheading:10742302-Receptor, Muscarinic M2,
pubmed-meshheading:10742302-Receptors, Muscarinic,
pubmed-meshheading:10742302-Tetrodotoxin,
pubmed-meshheading:10742302-Virulence Factors, Bordetella
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pubmed:year |
2000
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pubmed:articleTitle |
Functional role of muscarinic M(2) receptors in alpha,beta-methylene ATP induced, neurogenic contractions in guinea-pig ileum.
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pubmed:affiliation |
Department of Pharmacology, College of Medicine, University of California, Irvine, California, CA 92697-4625, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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