Linked Life Data

10742215

Source:http://linkedlifedata.com/resource/pubmed/id/10742215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-5-11
pubmed:abstractText
This paper reports on the effects of both reducing and nonreducing transgalactooligosaccharides (TOS) comprising 2 to 8 residues on the growth of Bifidobacterium adolescentis DSM 20083 and on the production of a novel beta-galactosidase (beta-Gal II). In cells grown on TOS, in addition to the lactose-degrading beta-Gal (beta-Gal I), another beta-Gal (beta-Gal II) was detected and it showed activity towards TOS but not towards lactose. beta-Gal II activity was at least 20-fold higher when cells were grown on TOS than when cells were grown on galactose, glucose, and lactose. Subsequently, the enzyme was purified from the cell extract of TOS-grown B. adolescentis by anion-exchange chromatography, adsorption chromatography, and size-exclusion chromatography. Beta-Gal II has apparent molecular masses of 350 and 89 kDa as judged by size-exclusion chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, respectively, indicating that the enzyme is active in vivo as a tetramer. Beta-Gal II had an optimal activity at pH 6 and was not active below pH 5. Its optimum temperature was 35 degrees C. The enzyme showed highest V(max) values towards galactooligosaccharides with a low degree of polymerization. This result is in agreement with the observation that during fermentation of TOS, the di- and trisaccharides were fermented first. Beta-Gal II was active towards beta-galactosyl residues that were 1-->4, 1-->6, 1-->3, and 1 <--> 1 linked, signifying its role in the metabolism of galactooligosaccharides by B. adolescentis.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-10230039, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-10508082, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-2666378, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-7782892, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-7811572, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-8006720, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-8398212, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-8640105, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-9082028, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-9114514, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-9871108, http://linkedlifedata.com/resource/pubmed/commentcorrection/10742215-9924286
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
66
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1379-84
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Characterization of a novel beta-galactosidase from Bifidobacterium adolescentis DSM 20083 active towards transgalactooligosaccharides.
pubmed:affiliation
Laboratories for Food Chemistry, Department of Food Technology and Nutritional Sciences, Wageningen University, Wageningen, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't